Characteristics of a potentiometric enzymatic biosensor based on a pH transducer for permethrin detection in treated wood are reported in this article. The transducer contained a plasticiser-free methacrylic-acrylic membrane immobilised with hydrogen ionophore and a lipophilic salt. A poly(2-hydroxyethyl methacrylate) (pHEMA) was used to immobilise carboxylesterase for the detection of permethrin. Linear response range of the biosensor based on the enzyme was 1.99–79.43 μM of permethrin (r2 = 0.9912). Limit of Detection (LOD) for the biosensor was 1.99 μM. This potentiometric enzyme based biosensor was specific to pyrethroid compounds and not organophosphate pesticides. Validation study was conducted to determine the accuracy of the permethrin determination using timber wood. The results showed that the enzyme-based biosensor was comparable to the HPLC method.

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