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Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty
The antigenic nature of Serpula lacrymans
1991 - IRG/WP 1492
The molecular nature of Serpula lacrymans has been extensively analysed by SDS-PAGE and distinctive banding patterns have been demonstrated. To develop simpler methods for identification of the organism an immunological analysis of a variety of isolates of both Serpula lacrymans and a number of other wood decay basidiomycetes has been undertaken. Results indicate unique antigenic profiles for Serp...
A Vigrow, H Glancy, J W Palfreyman, B King
Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King