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Non-enzymatic Gloeophyllum trabeum decay mechanisms: Further study
2001 - IRG/WP 01-10395
Information will be presented on the mechanisms involved in, and potential application of, non-enzymatic wood decay by brown rot decay fungi. Specifically, the hypothesized role of low molecular weight phenolate derivatives will be discussed in relation to non-enzymatic degradation of wood. The mechanism of binding of iron by cellulose, and binding and reduction of iron by fungal derivatives and m...
B Goodell, J Jellison


Preservatives treatment and field test monitoring of spruce pole stock: CCA and fumigant treatments
1990 - IRG/WP 3619
The fumigants trichloronitromethane (chloropicrin) and sodium N-methyldithiocarbamate (SNMDC) were used to treat red spruce pole stock, either CCA treated or untreated, through holes bored through the pole's center. The poles were analyzed for the presence of microorganisms immediately before ground installation and again after installation at a pole test site. Monitoring of fumigant move...
A J Pendlebury, B Goodell


The effect of low molecular weight chelators on iron chelation and free radical generation as studied by ESR measurement
2000 - IRG/WP 00-10367
The focus of this work was to improve our current knowledge of the non-enzymatic mechanisms involved in brown-rot decay. Electron Spin Resonance (ESR), also known as Electron Paramagnetic Resonance (EPR), is an attractive technique for the identification and study of chemical species containing unpaired electrons (such as radicals and certain transition metal species). ESR spin-trapping techniques...
Yuhui Qian, B Goodell


Wood degradation mechanisms by the brown rot fungus Gloeophyllum trabeum
1997 - IRG/WP 97-10229
A mechanism for the degradation of wood by the brown rot fungus Gloeophyllum trabeum is outlined. The mechanism includes the function of redox-cycling, low molecular weight phenolic derivatives which sequester and reduce iron in acidic environments. The role of oxalate for the sequestration of iron (hydr)oxides and the pH dependent transfer of iron to the G. trabeum phenolic chelators, as well as ...
B Goodell, J Jellison


Bordered Pit Imaging
2012 - IRG/WP 12-10773
New findings about bordered pits will be presented using the latest microscopy techniques. Three-dimensional imagery at the nanolevel is used, and short 3-D movies will shown as part of the discussion on this topic to reveal new features that have not previously been reported in pits. The implications for both microorganism penetration through lignified cells as well as preservative penetration i...
D Mascheck, B Goodell, H Militz, M Lessard, J Jellison


Inhibition of termite feeding by fungal siderophores
1992 - IRG/WP 92-1558
Siderophores are iron-chelating extracellular fungal metabolites which may be involved in initiating wood decay. A purified siderophore extract isolated from the brown-rot decay fungus Gloeophyllum trabeum (Pers. ex Fr.) Murr. (Basidiomycetes: Polyporaceae) was found to deter feeding by Formosan subterranean termites, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). This fungus has prev...
J K Grace, B Goodell, W E Jones, V Chandhoke, J Jellison


Effect of fumigant residue in aerated wood blocks on the spore germination of decay fungi
1991 - IRG/WP 2382
Fumigants are increasingly used in several countries for remedial treatments of transmission poles to increase the service life. The present study was initiated primarily to test the remaining toxic effects of spruce (Picea rubra) wood, fumigated with chloropicrin (trichloronitro methane) or MIT (Methyl isothiocyanate) after long period of aeration, on the spore germination of decay fungi. This st...
J Bjurman, B Goodell


Statistical evaluation of 'micro-bending' samples for classification of wood attacking fungi
1992 - IRG/WP 92-1544
Micro-specimens from both hardwood and softwood were exposed to selected species of field isolated fungi. The strength reduction and weight loss caused by these fungi were determined. A statistical procedure was then developed to classify the fungi into brown-, white-rot, or non-decay fungal categories based on these data. The procedure reveals the extent of hazard a fungus poses to wood members. ...
B Goodell, Jing Liu, A Homola, J Jellison, J Shottafer


Preservative treatment and field test monitoring of spruce pole stock: Pressure and diffusible chemical treatments
1990 - IRG/WP 3605
One hundred and forty four spruce (test species) and southern yellow pine (reference species) poles were variously treated by center boring, incising, or kerfing, followed by pressure treatment with CCA and/or diffusible preservatives. The diffusible preservatives included NaF/creosote, borax and ammonium bifluoride. The poles were set at a test site and evaluated for preservative distribution and...
B Goodell, A J Pendlebury


Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison


Cell wall microdistribution of chloropicrin and methylisothiocyanate in treated spruce
1989 - IRG/WP 3548
Chloropicrin and methylisothiocyanate (MIT) residues were observed using SEM/EDXA and TEM/EDXA in treated spruce wafers that had been exposed to the vapors of the two fumigant preservatives. Chlorinated residues from chloropicrin were found throughout the wood cell wall, even in acetone extracted material. The residues were most heavily concentrated in extractive materials in the rays, but unlike ...
G F Daniel, B Goodell


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Fungal siderophores and their rôle in wood biodegradation
1990 - IRG/WP 1442
Iron and other metals such as manganese, play an important role in the metabolic functions of fungi that cause wood deterioration. These transition metals are also found in, or associated with, the extracellular fungal enzymes shown to be directly involved in the decay process. Recently our research group was able to show that siderophores (low molecular weight biological chelators) are produced b...
J Jellison, B Goodell, F Fekete, V Chandhoke


Cyclic delamination analysis of preservative-treated wood/FRP interfaces
2002 - IRG/WP 02-40244
Wood preservative chemicals can interfere with adhesive properties when bonding wood laminates or wood/FRP (Fiber Reinforced Polymer) laminate composites. In this work we studied the effects of various wood preservative treatments, and pre- and post-treatment on wood/wood and wood/FRP bond durability using a severe cyclic delamination test (ASTM D-2559). Pre-treatment of individual laminates with ...
C Tascioglu, B Goodell, R Lopez-Anido


The effect of a chelator mediated fenton system on activation of TMP fibres and decolorization of synthesized dyes
2004 - IRG/WP 04-50223
The purpose of this work is to improve our current knowledge of the non-enzymatic mechanisms involved in the brown rot degradation of wood, but also to study the potential applications of a chelator-mediated Fenton system in activation of wood fibers and decolorization of synthesized dyes. In this work, Electron Spin Resonance (ESR) spin-trapping techniques were used to study the generation of hyd...
Yuhui Qian, B Goodell, J Jellison


Investigations into the biology of Meruliporia incrassata
2004 - IRG/WP 04-10508
The dry rot fungus Meruliporia incrassata (Berk. and Curt.) Murr. is a highly destructive brown rot wood decay fungus and is a significant pest of wooden structures. The fungus, know commonly as ‘Poria’, is characterized in culture by strand mycelium and skin-like surface mycelium. In structural environments it is found to produce prominent water conducting rhizomorphs, is a copious spore prod...
J Jellison, C Howell, B Goodell, S L Quarles


Environmentally benign wood preservatives based on organic biocide antioxidant combinations: A brief review of laboratory and field exposure results and discussion of a proposed mechanism
2004 - IRG/WP 04-30335
The combination of various organic biocides with commercial antioxidants generally increased the biocides’ efficacy 2-3 fold against wood-destroying fungi in short-term laboratory decay tests, and some positive results have been obtained after 2-4 years of outdoor exposure. The two antioxidants principally examined, propyl gallate and butylated hydroxytoluene, are low cost and, since both are f...
T Schultz, D D Nicholas, W Henry, C Pittman, D Wipf, B Goodell


The effects of preservative treatment and exposure to wood degrading fungi on fiber reinforced polymer (FRP) materials used for structural wood reinforcement
2001 - IRG/WP 01-40204
Glass fiber reinforced phenolic (GFRP) composite materials are becoming increasingly accepted for use in the construction industry because they combine advantages of both wood and advanced polymeric materials. Addition of only 1-3% FRP in the tension zone, for example, can typically improve the strength of the hybrid system by 200%. As more applications are found for wood/FRP hybrids, (e.g. lamina...
C Tascioglu, B Goodell


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Laser incising of spruce lumber for improved preservative penetration
1991 - IRG/WP 3646
Red spruce heartwood specimens were incised using a range of laser beam power levels and pulse durations. The penetration of CCA through these holes into the wood was monitored after pressure treatment. In addition, green and dried spruce samples were incised using two patterns with incision densities of either 16.1 holes/in² or 21.3 holes/in². Following CCA pressure treatment the samples were t...
B Goodell, F A Kamke, Jing Liu


Three-dimensional modeling of fumigant distribution in wood poles
1993 - IRG/WP 93-30025
The practice of using fumigants to control internal decay has been widely accepted in the United States and has shown to be effect in control internal decay in transmission poles and other large wood timbers. Mathematical models were developed to simulate the chloropicrin movement in utility poles after treatment. The simulation results indicated that treatment of utility poles, using either tradi...
Jing Liu, B Goodell


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


The effect of oil-borne preservative treatments on the shear strength of FRP/wood composite adhesive bonds
2003 - IRG/WP 03-40265
Reinforcement of structural wood components with Fiber Reinforced Polymer (FRP) will enhance the beam’s strength, but actual data on long-term durability is sparse, not well documented or not readily accessible. In this study, bond properties of FRP-wood composite materials were investigated following treatment with creosote or copper naphthenate preservatives. The properties investigated inclu...
B Herzog, B Goodell, R Lopez-Anido


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