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Molecular methods as a tool within the field of wood protection – available methods and new possibilities
2007 - IRG/WP 07-20361
The area of wood protection is in a period of change. New tools are needed to understand the mode of action, and to further improve the new wood protection systems. A set of useful tools are found among the molecular methods. This paper presents an overview of some of the tools available, and the methods are exemplified by papers within the frame of wood protection issues. However, there is stil...
G Alfredsen, A M Hietala, C G Fossdal, H Solheim


Postia placenta gene expression of oxidative and carbohydrate metabolism related genes during growth in furfurylated wood
2009 - IRG/WP 09-10701
A range of studies the last decade have shown that modified wood can provide excellent protection against a range of wood deteriorating organisms, including decay fungi. However, we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward e.g. inhibition of action of specific enzymes, but they still need testing. An understandi...
G Alfredsen, C G Fossdal


Postia placenta gene expression during growth in furfurylated wood
2010 - IRG/WP 10-10734
Modified wood can provide protection against a range of wood deteriorating organisms. But we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward for the mode of action against wood decaying fungi, including inhibition of action of specific enzymes, but they still need further testing. In this study gene expression of the b...
G Alfredsen, C G Fossdal


The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of ...
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz


Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and brown rot fungi are also largely responsible for the destructive decay of wooden structures. The aim of this study was to compare two commonly used strains of Postia placenta – MAD-698-R and FPRL 280. Scots pine sapwood samples were exposed for two and eight weeks to both fungal strains. The following was inv...
N Thaler, G Alfredsen, C G Fossdal


The effects of acetylation level on the growth of Postia placenta over 36 weeks
2012 - IRG/WP 12-40589
Genomic sequencing gives us a tool to systematically and rapidly discover novel genes, how their products function in the cell, and explore their interactions. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by different wood...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II