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Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty
Applications of immunological methods to the analysis and detection of Lentinus lepideus Fr
1989 - IRG/WP 1422
Polyclonal antisera have been raised against the brown rot fungus Lentinus lepideus, a major cause of rot induced pole failure in the UK. Specificity studies have indicated that the antisera cross-react with a number of basidiomycetes but to much lesser extent with other fungi. The antigenicity of Lentinus lepideus and hence its molecular composition shows some alteration with culture age and chan...
H Glancy, A Bruce, D Button, J W Palfreyman, B King
Molecular analysis of the basidiomycete Coniophora puteana
1992 - IRG/WP 92-1534
Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated org...
H E McDowell, D Button, J W Palfreyman
Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King