Your search resulted in 6 documents.
Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner
Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner
Reaction of osmium tetroxide with a diphenolic compound as a model substance for demethylated lignin
1986 - IRG/WP 1303
By using caffeic acid as a model substance for diphenolic moieties of demethylated lignin it was found that electron dense particles called by the authors "osmiophilic particles" in former papers are reaction products of OsO4 with the model substance. By these products it is possible to indicate the enzymatic demethylation of lignin taking place during wood decay. The appearance of the electron de...
K Messner, E Srebotnik
Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner
Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner
Degradation of polycyclic aromatic hydrocarbons by laccase
1998 - IRG/WP 98-10267
The degradation of polycyclic aromatic hydrocarbons (PAH) by laccase was investigated. Laccase catalyzed oxidation of phenanthrene, solubilized in the surfactant Tween 20, exhibited an absolute requirement for the mediator compound 1-hydroxybenzotriazole. When Tween 80 was used as the solubilizing agent, the oxidation rates were more than six times higher as compared to Tween 20. Using the thiobar...
S Böhmer, E Srebotnik, K Messner