Your search resulted in 140 documents. Displaying 25 entries per page.
Physical properties of ß-1,4-Xylanase produced by Postia (=Poria) placenta: Implications for the control of brown rot
1987 - IRG/WP 1318
The degradation of hemicelluloses is an early event in wood decay by brown-rot fungi. An understanding of the physical properties of hemicellulases may suggest target mechanisms for the development of new control agents. Endo-b-1,4-xylanase was partially purified by column chromatography from wood decayed by Postia (= Poria) placenta. The enzyme was extremely resistant to denaturing conditions; no...
J A Micales, F Green III, C A Clausen, T L Highley
Report of Section 1 2003
2003 - IRG/WP 03-10502
F Green III
Protection of Ochroma pyramidale from fungal decay with N,N-napthaloylhyroxylamine
1998 - IRG/WP 98-30182
Fungal decay of wood in service results in billions of dollars (U.S.) in losses annually. Recent environmental restrictions, both U.S. and international, are limiting and eliminating the use of broad-spectrum, heavy metal biocides for wood preservation. Restrictions result primarily from problems with disposal. New wood preservatives need to be developed and tested which specifically target key el...
F Green III, T L Highley
Oxalic acid production of fifteen brown-rot fungi in copper citrate- treated southern yellow pine
2001 - IRG/WP 01-10388
Non-arsenical copper-based wood preservatives have grown in number since the 1980's as a response to environmental concerns posed by arsenicals. Interest in copper tolerant decay fungi has increased accordingly. Oxalic acid (OA) production by brown-rot fungi has been proposed as one mechanism of copper tolerance. Fifteen brown-rot fungi representing the genera Postia, Wolfiporia, Serpula,...
F Green III, C A Clausen
Inhibition of wood decay and termite damage by calcium precipitation
1996 - IRG/WP 96-30111
Fungal decay of wood in service, especially brown rot, results in billions of dollars (US) of losses annually. Recent environmental restrictions, both U.S. and international, are limiting or eliminating the use of broad spectrum biocides for wood preservation, primarily due to problems with disposal. In order to design new, environmentally benign methods for control of wood decay fungi, it is esse...
F Green III, T A Kuster, L Ferge, T L Highley
Inhibition of termite damage by N'N-napthaloylhydroxyamine (NHA): Reticulotermes flavipes (Kollar) vs. Coptotermes formosanus Shiraki
2000 - IRG/WP 00-10354
The calcium precipitating agent NHA has been shown to protect southern yellow pine (SYP) from wood decay and termite damage comparable to CCA in field tests (Gulfport, MS) for two years (Crawford and Green, 1999). In a collaborative study, SYP wood blocks were vacuum treated with three concentrations of aqueous NHA and exposed in a no-choice test to Eastern subterranean termites (FPL, USA) and For...
F Green III, S T Lebow, T Yoshimura
Treatability problems - Relationships between anatomy, chemical composition and treatability
2001 - IRG/WP 01-40213
This report documents the results of phase 1 and 2 of a 3-phase research program. In phase 1, two hundred and fifty-six (256) Southern pine (pinus spp.) nominal 2 x 6's (38 mm x 140 mm) from a single mill in Georgia (southeastern US) were evaluated for treatability with CCA preservative. After treatment, 128 pieces representing a broad range of treatment characteristics were selected and ...
J E Winandy, F Green III, D Keefe
Evaluating the natural durability of native and tropical wood species against Reticulitermes flavip
2004 - IRG/WP 04-10539
Environmental pressures to eliminate arsenate from wood preservatives has resulted in voluntary removal of CCA for residential applications in the United States. A new generation of copper organic preservatives has been formulated to replace CCA for decking and in-ground applications but there is no guarantee that these preservatives represent a permanent solution to all related problems. There...
R A Arango, F Green III, K Hintz, R B Miller
Serpula lacrymans, The Dry Rot Fungus and its Tolerance towards Copper-based Wood Preservatives
2005 - IRG/WP 05-10555
Serpula lacrymans (Wulfen : Fries) Schröter, the dry rot fungus, is considered the most economically important wood decay fungus in temperate regions of the world i.e. northern Europe, Japan and Australia. Previously copper based wood preservatives were the most commonly used preservatives for pressure treatment of wood for building constructions. Because of a suspicion about tolerance toward cop...
A C Steenkjær Hastrup, F Green III, C A Clausen, B Jensen
Gas chromatographic determination of 1,8-naphthalimide, N-Hydroxy-1,8-naphthalimide (N,N-naphthaloylhydroxylamine) and the sodium salt of N-Hydroxy-1,8-naphthalimide
2003 - IRG/WP 03-20259
A number of naphthalimide (NI) derivatives are used as efficient laser dyes, in medicine or in scanning electron microscopy. Only N,N-naphthaloylhydroxylamine (NHA) has been shown to be an effective wood preservative against wood decay fungi and termite damage.
However, limited information is available concerning the analytical detection of NI-derivatives in treated timber. There is a clear need ...
E Melcher, F Green III
Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley
Early detection of brown-rot decay in southern yellow pine using immunodiagnostic procedures
1990 - IRG/WP 2356
Immunodiagnostic procedures have been used to detect incipient decay of southern yellow pine by six common brown-rot fungi. Rabbit polyclonal antibodies were raised to liquid culture fractions of the six fungi. The antibodies, after preadsorption to sawdust, were tested in particle agglutination assays, immuno-dot blot, and ELISA for their·ability to detect the decay organisms when they were grow...
C A Clausen, F Green III, T L Highley
Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III
Hemicellulosic induction of oxalic acid in Postia placenta
1994 - IRG/WP 94-10060
Most studies suggest that enzymes produced by brown-rot fungi are too large to penetrate sound wood structures, even after decay begins. Thus, nonenzymatic agents have been proposed to initiate brown-rot decay. We have reported that the brown-rot fungus Postia placenta MAD-698 initiates a 2-fold decrease in wood pH within 7 days of colonization which is mediated by production of oxalic acid. Strai...
F Green III, M Larsen, T L Highley
The long road to understanding brown-rot decay. A view from the ditch
1995 - IRG/WP 95-10101
Interest in understanding how brown-rot fungi decay wood has received increasing interest in recent years because of a need to identify novel targets that can be inhibited for the next generation of antifungal wood preservatives. Brown-rot fungi are unique in that they can degrade holocellulose (cellulose and hemicellulose) in wood without first removing the lignin. Furthermore, they degrade holoc...
F Green III, T L Highley
Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley
Mechanisms of Protection by NHA Against Fungal Decay
2002 - IRG/WP 02-10429
Treating wood with the water-borne sodium salt of N'-N-naphthaloylhydroxylamine (Na-NHA) protects wood against decay and termite damage. Initial testing indicated little or no inhibition of sapstain fungi, molds, or soft-rot fungi by Na-NHA, suggesting that the
mechanism by which this compound protected wood was complex and not that of a broad-spectrum biocide. Previously, we (Green et...
F Green III, W Henry, T Schultz
Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster
Detection of increased metal cations after wood decay using Chromeazurol-S
1997 - IRG/WP 97-20112
Chromeazurol-S (CAS) is a dye used for the spectrophotometric determination of metals, mainly aluminum (Al), berillium (Be) and copper (Cu). CAS has been widely used for determining the penetration of copper containing preservatives like CCA (AWPA A3-96). Additional uses include: i) detection of utility pole decay (Esyln, 1979), ii) a chemical spot test for aluminum in wood (Kukachka and Miller, 1...
F Green III, U Srinivasan, R B Miller
Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley
Accidental mold/termite testing of high density fiberboard (HDF) treated with borates and N’N-naphthaloylhydroxylamine (NHA)
2003 - IRG/WP 03-10462
High density fibreboard (HDF) was made from beech and pine furnish (50:50) and treated with boric acid (0.1-3%), borax (0.1-3%) or N'-N-(1,8-naphthalyl) hydroxylamine (NHA) (0.1-1%) prior to gluing with urea formaldehyde (UF) resin in order to determine resistance to Eastern subterranean termites (Reticulitermes flavipes Kollar), the most economically important termite species in North Am...
S N Kartal, H H Burdsall Jr, F Green III
Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley
Distribution of boron from fused borate rods in Douglas-fir transmission poles
1996 - IRG/WP 96-30112
The diffusion of boron from fused borate rods (disodium octaborate tetrachydrate) was monitored over 42 months in CCA-treated Douglas-fir transmission poles. The boric acid equivalent was estimated by the curcumin/salicylic acid color test on increment cores removed from the poles. Moisture content of the poles was quite variable but was always above 20 percent. The percent of increment core lengt...
T L Highley, F Green III, W F Finney
Borate diffusion from fused borate rods in douglas-fir transmission poles
1994 - IRG/WP 94-30042
Pressure-treated utility poles have given many years of excellent service, but their lifetime is often shortened by internal decay of the untreated heartwood center, particularly in refractory species, such as Douglas-fir. This paper reports the distribution of boron from fused borate rods installed in CCA-treated Douglas-fir transmission poles. The boric acid equivalent was roughly monitored by t...
T L Highley, W Finney, F Green III
The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley