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Detection of semi-quantitative and qualitative enzymatic activities of blue-stain fungi
2000 - IRG/WP 00-10347
Blue-stain, produced in forests, continues to be a problem in countries of moderate climate. In forestry, in certain geographical areas of Spain, it has been observed that anti-sapstain products have not always been efficient, as their fungicidal effectiveness varies on occasion, depending on the species of wood and microbiota that exist in the region. It is not always easy to identify the species...
M T De Troya, F Llinares, D Muñoz-Mingarro, M J Pozuelo, N Acero, C Rodríguez-Borrajo, A M Navarrete


Biocontrol and bioprotection of wood decay and sapstain fungi
2003 - IRG/WP 03-10474
The most serious damages caused in the wood industries by decay and sapstain fungi, and the growing international pressures on the use of biocides products with toxic active ingredients harmful to human health and the environment, have made it necessary to search for alternative chemical products to protect the wood, and for the possibility to use micro-organisms as biological controllers. This la...
M T De Troya, F Rubio, M Villarreal, F Arenal, F Llinares, V Rubio, D Muñoz-Mingarro, N Acero


Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10210
In this work, the in vitro species of Pleurotus (P.eringii, P. cornucopiae and P. ostreatus), from structures of degraded wood have been studied. This degradative activity was compared to those presented by Trametes versicolor (fungus characteristic of white rot) and Gloeophyllum trabeum (fungus representative of brown rot). The activities studied were the following: cellulase, xylanase, pectinase...
D Muñoz-Mingarro, F Llinares, M T De Troya, F Rubio, M Yuste, C Rodríguez-Borrajo, J E Garcia de los Rios, F Alvarez, P Jiménez, A Rojas, A Navarrete, P Reche


Comparative study of the degradative ability of autochthonous and standardized strains of wood decay fungi
1996 - IRG/WP 96-10178
This study is intended to assess the biomass, the extracellular protein production, and the carbohydrate degradating ability of three standarized (Chaetomium globosum, Trametes versicolor and Gloeophyllum trabeum) and six autochthonous wood decay fungi strains, with unknown degradating effects. The carbohydrate sources used were those that are usually found in wood cells (glucose, galactose, manos...
F Llinares, D Muñoz-Mingarro, J E Garcia de los Rios, M T De Troya, M Yuste, F Rubio, F Alvarez, P Jiménez, C Rodríguez-Borrajo, A M Navarrete


Proposal for a simple methodology for the evaluation of the preventive effectiveness of protectors applied in superficial treatments against basidiomycetes fungi
1999 - IRG/WP 99-20167
Due to the considerable effect that the relation between the lateral surface and the volume of wood blocks has on superficial treatments, this work has attempted to determine the ideal size which allows to evaluate the effectiveness of a preservative in the shortest possible time. Three wood blocks of different dimensions were tested. In addition, as it is necessary to neutralise the effect of the...
M T De Troya, A Navarrete, F Rubio, M Yuste, C Rodríguez-Borrajo, D Muñoz-Mingarro, F Llinares


Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete


An alternative method for the determination of lignocellulolytic enzymatic activities of white rot fungi
1998 - IRG/WP 98-10261
Some lignocellulolytic activities of wood decay fungi can be evaluated in an indirect way through the study of the decolorization that white rot fungi produces in culture media in the presence of dyes. In this assay, ten dyes were selected, which were added in concentrations of 0.00 1% - 0.003% (P/V) to four-day cultures of Trametes versicolor containing 1% of beech sawdust in a basal salt medium...
D Muñoz-Mingarro, F Llinares, F Rubio, M Yuste, C Rodríguez-Borrajo, J Jiménez, M T De Troya


Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín


Influence of the nature of the substrate and the method of inoculation on the degradative activity of soft rot fungi
1998 - IRG/WP 98-20133
The European Standard (ENV-807) determines the threshold of effectiveness of wood preservatives against the action of soft rot Ascomycetes fungi. Its application in the treatment of wood used in the hazard class 4 does not always offer repeatable results, which could be expected in an experimental norm. For this reason, in this paper, we have evaluated the diverse variables that might influence t...
M T De Troya, A M Navarrete, F Rubio, J Jiménez, D Muñoz-Mingarro, C Rodríguez-Borrajo, F Llinares, M Yuste


Study of the durability of the Maderon® against wood decay fungi and insects
2011 - IRG/WP 11-40548
Many industries produce residues that are not easily degradable. One of these is nut husks which, due to their high level of resistance to biological and environmental degradation, have become an ecological problem. Maderón® is a material invented and patented in Spain employed as an ecological substitute and a modern alternative to certain uses of wood. At present, Maderón® is commercialized ...
M T Troya, F Llinares, P Jiménez, J I Fernández-Golfín, M Conde, R Díaz


Wood decay enzymatic ability of Fusarium circinatum
2012 - IRG/WP 12-10777
There exists pathogens that attack standing trees of forests, and do not usually thrive once the tree is felled. However, occasionally they can be present in a latent state in the wood and can spread and affect new areas with economic, ecological and social consequences. Fusarium circinatum is a fungus that causes “pitch canker” in pine species, and is considered a quarantine organism within t...
M T Troya, F Llinares, Y Serrano, M Conde


Survival of Bursaphelenchus xylophilus exposed to permethrins
2012 - IRG/WP 12-30585
The pine wilt nematode Bursaphelenchus xylophilus (Steiner and Buhrer 1934) Nickle, 1970 is a quarantine organism with a high potential to damage Conifer forests in Europe. This has driven the European Union to protect forest stands from the propagation and dispersion of this pathogen.The nematode was first detected in Europe in Setubal (Portugal) in 1999 and eradication measures were taken. Howev...
S C Arcos, M T Troya, L Robertson, F Llinares, M Conde, A Navas


Survival of Bursaphelenchus xylophilus exposed to different active ingredients of wood protectors
2013 - IRG/WP 13-10802
The pine wilt nematode (PWN) Bursaphelenchus xylophilus (Steiner and Buhrer 1934) Nickle, 1970 is a quarantine organism with a high potential to damage Conifer forests in Europe. This has driven the European Union to protect forest stands from the propagation and dispersion of this pathogen.The nematode was first detected in Europe in Setubal (Portugal) in 1999 and eradication measures were taken....
S C Arcos, M T Troya, L Robertson, F Llinares, M Conde, A Navas


Product efficacy against Fusarium circinatum, a quarantine organism in Europe
2013 - IRG/WP 13-10807
Fusarium circinatum Nirenberg et O'Donnell (telemorph: Gibberella circinata Nirenberg et O'Donnell) is a quarantine organism in Europe producing the disease known as pitch canker of pines. For a decade, the European Union has taken steps to locate and eradicate the pathogen with the aim of eliminating the risk of spread. So far, research has focused on forest stands, however, the movemen...
M T Troya, R Raposo, F Llinares, M Conde


Efficacy of commercial wood preservatives against Bursaphelenchus xylophilus, a quarantine organism in Europe
2014 - IRG/WP 14-30638
Bursaphelenchus xylophilus (Steiner and Buhrer 1934) Nickle, 1970, pine wilt nematode (PWN), is a quarantine organism with a high potential to damage Conifer forests in Europe. This has driven the European Union to protect forest stands from the propagation and dispersion of this pathogen. Since the nematode was detected in Europe, eradication measures were taken. However, these measures were unsu...
C S Arcos, A Navas, L Robertson, M T Troya, M Conde, F Llinares


Study of the effectiveness of wood products for permanent protection against Fusarium circinatum
2014 - IRG/WP 14-30655
In Europe there exist a number of quarantine organisms that affect conifer forests, including Fusarium circinatum Nirenberg et O'Donnell (telemorph: Gibberella circinata Nirenberg et O'Donnell), a quarantine organism in Europe producing the disease known as pitch canker of pines. For a decade, the European Union has taken steps to locate and eradicate the pathogen with the aim of elimina...
M T Troya, M Conde, R Raposo, F Llinares, M Elvira-Recuenco


Influence of the moon phase on durability against wood decay fungi of Larix kaempferi and Pseudotsuga menziesii wood species
2015 - IRG/WP 15-10836
The analysis of the natural durability of wood is essential when establishing the level of protection required, depending on the designated end use of timber. On the other hand, there is a popular belief based on the influence of the moon phase in felling timber, recommending the waning moon as the optimum for felling trees. In fact, some companies now offer among their products, sawn timber felle...
M T Troya, M Conde, M J Barriola, F Llinares, V Dorronsoro


Wood protectors to control Bursaphelenchus xylophilus
2015 - IRG/WP 15-30661
Bursaphelenchus xylophilus, the pine wilt nematode (PWN), is a quarantine organism with a high potential to damage Conifer forests in Europe. The European Union is concerned in the protection of forest stands from the propagation and dispersion of this pathogen. In this line, the current control measures have been unsuccessful in preventing the dispersion of this organism, as it continues to be de...
M T Troya, C S Arcos, A Navas, L Robertson, M Conde, F Llinares


Durability to decay organisms of Pinus nigra subsp. laricio var. corsicana. Influence of the moon phase
2016 - IRG/WP 16-10869
Actually the use of wood has an added value since it is a renewable, biodegradable and recyclable material. For this reason, the evaluation of it natural durability is essential when establishing the level of protection required, being depending on the designated end use of timber. On the other hand, there is a popular belief based on the influence of the moon phase in felling timber, recommending...
M T Troya, M Conde, M J Barriola, F Llinares, V Dorronsoro


Is the SPM 15 effective? Survival of Pine Wood Nematode under thermal treatments
2017 - IRG/WP 17-40775
The pine wood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (PWN) is the causal agent of “Pine wilt disease” affecting coniferous forests, and since its detection in 1999 in Portugal, has obligated the European Union to take action to prevent its spread (Decision 2006/133/CE). The main measure applied is the International Standard for Phytosanitary Measures ISPM No. 15 (ISPM ...
L Robertson, M Conde, J C Cabrero, F Llinares, M T Troya


Biocontrol of wood decay insects and nematodes as an alternative to traditional chemical treatments
2018 - IRG/WP 18-10914
The protection of wood has been defined until relatively recently, as the application of a chemical to increase its durability. On the other hand, society is becoming more and more demanding in terms of the use of products which have the minimum ecological impact. This has led many countries to reduce the list of biocidal products that can be used (European Biocides Regulation), therefore it is ne...
L Robertson, J F Galván, F Llinares, R Viñambres, M T de Troya


Efficacy of microwave treatments against Pine Wood Nematode
2019 - IRG/WP 19-20658
The pine wood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (PWN) is the causal agent of “Pine wilt disease” affecting coniferous forests, and since its detection in 1999 in Portugal, has obliged the European Union to take action to prevent its spread (Decision 2006/133/CE). One of the main measures applied is the International Standard for Phytosanitary Measures ISPM No. 15 ...
L Robertson, M T Troya, F Llinares, J C Cabrero


Role of microbiota in wood degradation by Reticulitermes grassei and Bursaphelenchus xylophilus
2022 - IRG/WP 22-20684
Xylophagous organisms can cause damage both in forests and in felled wood. Bursaphelenchus xylophilus, which causes "Sudden Pine Wilting" in coniferous forest masses, and is currently considered a quarantine organism in the European Union. On the other hand, structural and carpentry wood is affected by subterranean termites (Reticulitermes spp.), that cause serious damage, both in buildings and fu...
L Robertson, S Rames, M Uriel, J M González, F Llinares, S M Santos, M T Troya


Effect of enzymatic inhibitors on the population of Reticulitermes grassei and their associated microbiota
2023 - IRG/WP 23-11008
Termites are pests that invade urban, agricultural and forest environments worldwide. They are economically important owing to the damage they cause to wood, wood products, building materials, agricultural products and forests. There are many methods for its control, but many of them cause negative environmental effects due to their toxicity. In termites, dietary change or antibiotic treatment may...
S M Santos, M T Troya, F Llinares, P Colina, E García, M Álvarez, L Robertson


Isolation of the microbiota from wood decay insects and some of their enzymatic activities
2023 - IRG/WP 23-11014
Wood decay organisms have the ability to degrade wood components (cellulose, hemicellulose and lignin) by enzymatic capacities, but these enzymes can belong to the proper organism, or to the associated microbiota. The identification of the microorganisms, as well as their role in the digestion of many substrates not always is very well known. The wood decay insects produce big damages in wood str...
M T Troya, F Llinares, J Arranz, S Izpura-Luis, S M Santos, L Robertson