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The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum
1990 - IRG/WP 1447
The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extrac...
A-C Ritschkoff, M Rättö, L Viikari


Fenton's reagent as a modification tool in brown-rot decay
1996 - IRG/WP 96-10155
A biomimetic approach was used to clarify the role and importance of the Fenton-type reaction in the carbohydrate degradation by brown-rot fungi. Spruce sawdust and microcrystalline cellulose were modified in the H2O2/Fe(II) treatment. The degree of hydrolysis of the pretreated spruce sawdust was clearly increased with the complete cellulase (Econase), purified endoglucanase from Trichoderma reese...
M Rättö, A-C Ritschkoff, J Buchert, L Viikari


Production of exraxellular hydrogen peroxide and oxalic acid by brown-rot fungus Poria placenta
1995 - IRG/WP 95-10112
Hydrogen peroxide and oxalic acid have been suggested to be essential in the degradation of wood carbohydrates by brown-rot fungi. Hydrogen peroxide has been suggested to be one of the diffusible low molecular weight agents produced by brown-rot fungi for the degradation of wood carbohydrates by generating highly active radicals. The production of hydrogen peroxide and oxalic acid by Poria placent...
A-C Ritschkoff, M Rättö, J Buchert, L Viikari


Effect of some resin treatments on fungal degradation reactions
1999 - IRG/WP 99-10318
The effect of impregnation of wood with vegetable oil, tall oil and two water soluble on fungal degradation reactions was assessed by hydrolysis and enzyme activity measurements. The experiments were carried out by using isolated enzyme preparations and a living brown-rot fungus Poria placenta. The results indicate that the inhibitory effect of treatments was clearly directec towards the enzymatic...
A-C Ritschkoff, M Rättö, A J Nurmi, H Kokko, A O Rapp, H Militz


Influence of the nutritional elements on pigmentation and production of biomass of bluestain fungus Aureobasidium pullulans
1997 - IRG/WP 97-10198
The effect of the carbon source and the amount of the nitrogen on the melanization and the production of mycelial mass of bluestain fungus Aureobasidium pullulans was studied by using solid state cultivations. The carbon sources used varied from easily soluble sugars to structural polysaccharides existing in lignocellulosic material. The amount of melanin was evaluated by using partial purificatio...
A-C Ritschkoff, M Rättö, F Thomassin