Your search resulted in 12 documents.
Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt
A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl
Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl
Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl
Characterization of protein patterns from decayed wood of loblolly pine (Pinus taeda L.) by proteomic analysis
2008 - IRG/WP 08-10654
The primary biotic decomposers of wood belong to the basidiomycetes. The members of this group can attack and biodegrade both wood in the forest and in service. By the time wood decay is visible, there has already been a significant loss of strength. The identification of basidiomycetes and other organisms on wood only tell us what is present, not what is actively decaying the wood. When organisms...
Young-Min Kang, L Prewitt, S Diehl
Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl
Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang
Gene expression of selected decay enzymes produced during biodeterioration of three wood types
2009 - IRG/WP 09-10702
Comparative studies were conducted on the expression of several decay enzyme genes during the decay of pine, cedar, and ACQ treated pine over 10 months. Measurements of MOE, decay rating, and moisture control were monitored for physical properties. Identification of decay fungi and the detection of decay enzymes were carried out before gene expression levels were measured. The MOE of wood stakes d...
Young-Min Kang, L Prewitt, S Diehl
Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas
Remediation of Pentachlorophenol (PCP) in Biodiesel versus Diesel Carriers
2011 - IRG/WP 11-50279
Biodiesel is believed to be more environmentally friendly than petroleum based diesel when used as a carrier for impregnation of PCP into wood products for protecting wood from decay. A six month study was conducted to evaluate the remediation of PCP in biodiesel versus diesel in soil. Different percentages of biodiesel, diesel and PCP were mixed with clean soil from a forested site and tested. Sa...
S Keshani Langroodi, H Borazjani, D Nicholas, L Prewitt, S V Diehl, S Kitchens
Effect of Biodiesel on the Efficacy of Pentachlorophenol Treated Wood
2012 - IRG/WP 12-30584
Pentachlorophenol (PCP) is widely used as a wood preservative for utility poles and other wood products. Recently, it has been proposed that a modified PCP carrier system based on a diesel/biodiesel mixture should be used in place of the conventional diesel/KB3 carrier, but there is some question as to whether or not this modified carrier system can provide the same service life for wood products ...
S Keshani Langroodi, H Borazjani, D Nicholas, L Prewitt, S V Diehl
Bacterial Degradation of Pentachlorophenol Indicated in Groundwater Undergoing Long Term In-Situ Remediation
2016 - IRG/WP 16-50318
Pentachlorophenol (PCP, Penta) is a worldwide soil and water contaminant. The pervasive use of PCP as a fungicide, herbicide, pesticide, and as preservative for wood products has led to its global distribution. It is a highly effective preventative measure for most invasive insects, bacteria, fungi, and molds, as well as an excellent wood protection treatment. This study examined a known PCP conta...
C E Stokes, H Borazjani, L Prewitt