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Synergistic effect of boron on Streptomyces rimosus metabolites in preventing conidial germination of sapstain and mold fungi
1992 - IRG/WP 92-1565
We evaluated the synergistic effect of boron (4% BAE solution of Tim-Bor or 4% boric acid) on Streptomyces rimosus metabolites in preventing spore germination of sapstain and mold fungi using plate bioassay, Southern yellow pine and sweetgum block tests, and green pine log sections: sapstain -- Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans; mold fungi -- Aspergillus nig...
S C Croan, T L Highley


Basidiosporogenesis by the white-rot basidiomycetes in vitr
1994 - IRG/WP 94-10081
Basidiospores of all forest-inhabiting basidiomycetes are a primary source of infection causing wood decay. However, most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the white-rot basidiomycetes, Schizophyllum commune and Tr...
S C Croan


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia car...
S C Croan


Bioconversion of wood wastes into gourmet and medicinal mushrooms
1999 - IRG/WP 99-50129
Increased wood wastes, including thinned material from stagnated and overstocked small-diameter forests, are a menace to forest health, to the sustainability of ecosystems, and to community economic viability. The objective of this study is to recycle wood wastes into value-added products, such as gourmet and medicinal mushrooms, by using the white-rot basidiomycetes, Pleurotus ostreatus, P. popul...
S C Croan


Wood extractive concentration and sem examination of pretreated southern yellow pine wood chips with blue-stain fungi for mushroom production
2001 - IRG/WP 01-10407
Mushroom-producing white-rotting basidiomycetes either do not colonize or else colonize very poorly on freshly prepared southern yellow pine wood chips. This study evaluates the resinous extractive content of southern yellow pine before and after treatment with colorless mutant blue-stain fungi. The blue-stain fungi penetrate into the sapwood of southern yellow pine and utilize nonstructural resin...
S C Croan, J Haight


Preservation of hyphal-forming brown- and white-rot wood-inhabiting basidiomycetes
2001 - IRG/WP 01-10397
Lyophilization is an excellent technique for the long-term preservation of hyphal-forming brown- and white-rot wood-inhabiting basidiomycotina. However, vegetative mycelial isolates are not lyophilizable. In this study, 10 brown-rot and 10 white-rot basidiomycetous non-sporulating, non-chlamydosporulating, and non-bubillerferous basidiomycetes fungi were tested for viability after lyophilization. ...
S C Croan


Biological control of sapstain fungi in wood
1996 - IRG/WP 96-10158
Sapstain fungi can cause serious damage to wood and wood products, resulting in a significant economic loss for the wood products industry. The purpose of the present investigation was to determine whether the sapstain in wood caused by sapstain fungi could be biologically controlled. Biological control of sapstain fungi in wood was demonstrated in field trials with nonpigmented isolates of Cerato...
S C Croan


Biosynthesis of ß-Glucan microfibrils by cellular fractions from brown-rot fungus Postia placenta (MAD-698 and ME-20) and white-rot fungus Schizophyllum commune (MAD-619)
1993 - IRG/WP 93-10025
In this study, we compared the brown rot fungus Postia placenta (MAD-698 and ME-20) with the white rot fungus Schizophyllum commune (MAD-619) to determine the location and distribution of glucan synthetase. We also measured the soluble protein content in subcellular fractions obtained by differential centrifugation MAD-698 is a degradative isolate, but ME-20 and MAD-619 do not produce significant ...
S C Croan, T L Highley


Controlling the sapstain fungus Ceratocystis coerulescens by metabolites obtained from Bjerkandera adusta and Talaromyces flavus
1993 - IRG/WP 93-10024
Sapstain causes severe damage to wood and wood products, posing a major economic problem for the wood industry. The purpose of this study was to determine if metabolites from Bjerkandera adusta and Talaromyces flavus would (1) decolorize stain in wood caused by Ceratocystis coerulescens and (2) prevent sapstain by Ceratocystis coerulescens. We studied the interaction of the sapstain fungus Ceratoc...
S C Croan, T L Highley


Environmentally benign biological wood preservatives by Streptomyces rimosus, SC-36
1997 - IRG/WP 97-10196
Microbiocides obtained from mutant culture Streptomyces rimosus SC-36 were evaluated for their abilities to inhibit the growth of brown-rot, soft-rot, white-rot, and sapstain fungi on wood. The test fungi were the brown-rot fungi Antrodia carbonica, Gloeophyllum trabeum, Neolentinus lepideus, and Postia placenta; soft-rot fungi Chaetomium globosum, Paecilomyces variotti, Phialocephala dimorphospor...
S C Croan


Destaining wood sapstains caused by Ceratocystis coerulescens
1996 - IRG/WP 96-10159
Fungal sapstain does not reduce the strength of wood, but it does discolor the wood, detracting from its appearance and decreasing the value of wood and wood products. The purpose of this investigation was to assess whether wood sapstain caused by Ceratocystis coerulescens could be destained and existing growth eradicated. The hydroxyl radicals generation under optimized conditions destained the s...
S C Croan


Control of sapwood-inhabiting fungi by fractionated extracellular metabolites from Coniophora puteana
1991 - IRG/WP 1494
The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicillium spp. The acetone-soluble fraction obtained from the culture filtrate prepared from Coniophora puteana grown on 6% malt extract agar inhibi...
S C Croan, T L Highley


Laboratory studies on control of sapstain and mold on unseasoned wood by bacteria
1991 - IRG/WP 1493
A malt-agar and nonagar laboratory test was used to evaluate the efficacy of bacterial preparations as biological control agents against several sapwood-inhabiting fungi. Both nonsterilized and filter sterilized bacteria preparations prevented attack by the sapwood-inhabiting fungi. Streptomyces rimosus was the most effective bacteria, giving good protection, with a 1-hr soak in a filter-sterilize...
T L Highley, R Benko, S C Croan


Antagonistic effect of Trichoderma spp. against basidiospores
1993 - IRG/WP 93-10027
All screening of potential biocontrol agents of wood decay fungi have previously been used against basidiomycete mycelium. Similarly, experiments designed to evaluate the mechanisms involved in antagonism (soluble metabolites and volatile antibiotics) between biological control agents and target fungi have always been carried out on mycelial inoculum. Basidiospores are a primary source of infectio...
U Srinivasan, T L Highley, S C Croan, A Bruce


Effect of thickened boron in preventing conidial germination of sapwood-inhabiting fungi
1994 - IRG/WP 94-30059
We evaluated the efficacy of thickened boron preservative "Diffusolä" in preventing conidia germination of sapwood-inhabiting fungi using plate bioassay, Southern Yellow Pine and sweetgum block tests, and green pine log sections. The test fungi were sapstain fungi Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans and mold fungi, Aspergillus niger, Penicillium spp, and Tric...
S C Croan


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Biological control of sapwood-inhabiting fungi by living bacterial cells of Streptomyces rimosus as a bioprotectant
1992 - IRG/WP 92-1564
The objective of this study is to evaluate the efficacy of antifungal activity of living bacterial cells for the protection of wood against sapwood-inhabiting fungi. The following sapwood-inhabiting fungi were selected: sapstain --Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi --Aspergillus niger, Penicillium spp, and Trichoderma spp. L...
S C Croan, T L Highley


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Antifungal activity in metabolites from Streptomyces rimosus
1991 - IRG/WP 1495
The objective of this study was to evaluate the efficacy of antifungal metabolites from Streptomyces rimosus for controlling the growth of sapwood-inhabiting fungi: sapstain fungi - Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi - Aspergillus niger, Penicillium spp, and Trichoderma spp. Production of antifungal metabolites by Streptomyc...
S C Croan, T L Highley