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Distribution of cellulases in the body of Coptotermes formosanus and the probability that the termite uses glucose as an energy and carbon sources
1997 - IRG/WP 97-10202
We assayed extracts of the digestive system and of the whole body of Coptotermes formosanus to determine where the various cellulases, glucose, and related substances were concentrated and to detect pyruvate dehydrogenase activity in the hindgut-removed body in order to verify its full cellulolytic system. About 20%, 18% and 36% of the total exo-1,4-ß-glucanase activity of C. formosanus were dete...
S Itakura, H Tanaka, A Enoki


Characterization of glycopeptide from white-, brown- and soft rot fungi
2002 - IRG/WP 02-10424
Extracellular low-molecular-weight substances that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radicals have been isolated from wood degrading cultures of white-rot, brown-rot, and soft-rot fungi. They contained protein, neutral carbohydrate, and Fe(II). These substances were glycosylated (glycated) peptides and suggested to form Amadori product (ketoamine). The...
H Tanaka, S Itakura, A Enoki


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


Wood and filter paper degradation, phenol oxidase and one-electron oxidation activities by the white rot fungus Ceriporiopsis subvermispora
2003 - IRG/WP 03-10486
The activities of one-electron oxidation and phenol oxidase during incubation of cultures of the white-rot basidiomycete Ceriporiopsis subvermispora containing either glucose or wood were periodically measured. Further, the degradation activities against wood and filter paper were examined during the course of cultivation. Weight losses of Japanese beech wood and Japanese cedar wood after 12 wee...
H Tanaka, S Itakura, A Enoki


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


The involvement of hydroxyl-radical-producing glycoprotein from the white-rot basidiomycete Ceriporiopsis subvermispora in wood decay
2009 - IRG/WP 09-10688
White-rot decay can be divided into two subtypes. One type involves the simultaneous degradation of all wood components through the formation of erosion troughs with a progressive thinning of wood cell walls. This type of decay is consistent with a model in which several polymer-degrading enzymes act on the exposed surfaces of the wood cell walls, producing progressive erosion from the lumen to th...
H Tanaka, Y Inoue, T Morikawa, S Itakura, A Enoki