IRG Documents Database and Compendium


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Confocal laser scanning microscopy of a novel decay in preservative treated radiata pine in wet acidic soils
1997 - IRG/WP 97-10215
Light microscopy of radiata pine (Pinus radiata D. Don) field test stakes (20x20x500mm3) exposed in wet acidic (pH 3-4) soil for 12 - 24 months showed predominance of an unusual type of decay characte-rised by tunnelling attack of wood cell walls. After two years decay was moderate to severe in wood treated to ground contact CCA specifications and also equivalent retentions of creosote, and a numb...
R N Wakeling, Ying Xiao, A P Singh


Fungal and bacterial attack of CCA-treated Pinus radiata timbers from a water-cooling tower
1991 - IRG/WP 1488
Transmission electron microscopy of decaying CCA-treated Pinus radiata timbers from an industrial water cooling tower showed presence of a thick biofilm covering some areas of the wood. The biofilm contained various morphologically distinct forms of microorganisms embedded in a slime. The study provided evidence of the activity of soft rot fungi and tunnelling and erosion bacteria in wood cells. T...
A P Singh, M E Hedley, D R Page, C S Han, K Atisongkroh


Resistance of Alstonia scholaris vestures to degradation by tunnelling bacteria
1992 - IRG/WP 92-1547
Electron microscopic examination of vessels and fibre-tracheids in the wood of Alstonia scholaris exposed to tunnelling bacteria (TB) in a liquid culture showed degradation of all areas of the secondary wall. The highly lignified middle lamella was also degraded in advanced stages of TB attack. However, vestured pit membranes and vestures appeared to be resistant to degradation by TB even when oth...
A P Singh, T Nilsson, G F Daniel


Ultrastructure of the attack of a naturally durable timber by tunnelling bacteria
1990 - IRG/WP 1462
The attack of the wood of Eusideroxylon zwageri, a naturally durable species, by tunnelling bacteria (TB) was examined by light microscopy and transmission electron microscopy. Observations were made primarily on fibres. Parenchyma were included in some cases. Both fibres and parenchyma are rich in extractives. In fibres, extractives are primarily present in the lumen. The amber colouration of fib...
A P Singh, T Nilsson, G F Daniel


Procedure for a collaborative study of leaching from CCA-treated timber in the sea
1988 - IRG/WP 4143
As a follow-up to the collaborative IRG/COIPM marine trial of CCA and CCB preservatives, a further trial is proposed with the objective of measuring the degree of leaching of CCA components from three timber species at marine sites in Malaysia and the U.K.. Details of the planned procedure are given....
S M Cragg, J E Barnacle, J D Bultman, R A Eaton, B R Johnson, L E Leightley, K D Singh


The true dry rot fungus (Serpula lacrymans) found in the wild in the forests of the Himalayas
1993 - IRG/WP 93-10002
J Bech-Andersen, S A Elborne, F Goldie, J Singh, B Walker


Microscopic characteristics of Pinus radiata wood veneers weathered outdoors for one yea
1993 - IRG/WP 93-10018
Radiata pine (Pinus radiata) wood veneers glued to solid wood hacking strips were exposed outdoors at 45° north-facing for one year and then examined by light microscopy (LM) and transmission electron microscopy (TEM). As expected, the exposed wood surfaces were colonised by a variety of microorganisms; however, wood decay was not observed. Ultlaviolet (UV light) radiation and water were the main...
A P Singh, R A B Sweeney, E A Dunningham, D V Plackett


Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


In vitro studies on the effect of chitosan on mycelial growth and spore germination of decay fungi, moulds and staining fungi
2004 - IRG/WP 04-10507
The effect of solubilised, low molecular weight chitosan on established mycelial growth of a range of decay fungi, moulds and staining fungi was investigated using nutrient medium amended with different concentrations of chitosan that ranged from 0.1 to 0.4% weight per volume (%w/v). Also, spore germination of Trichoderma harzianum and Leptographium procerum was examined on chitosan amended nutrie...
C Chittenden, B Kreber, N McDowell, T Singh


Degradation of the normal fibre walls of rubberwood (Hevea brasiliensis) by the tropical blue-stain fungus Botryodiplodia theobromae
1998 - IRG/WP 98-10286
Rubberwood was examined by light microscopy and transmission electron microscopy (TEM) after exposure to the common tropical sapstain fungus Botryodiplodia theobromae for four weeks to study hyphal colonisation of wood cells and to determine if this fungus also degraded lignified normal fibre cell walls in addition to the walls of non-lignified elements. Light microscopy revealed relatively large ...
A A H Wong, A P Singh


Isolation of the dry rot fungus, Serpula lacrymans, from the forests of the Himalayan Foothills
1995 - IRG/WP 95-10129
Previous expeditions to the Himalayas (Singh 1993, 1994) have reported on the presence in this area of the dry rot fungus Serpula lacrymans. However, attempts to isolate the organism from material brought back from these expeditions were not successful. In this paper we report on the isolation of Serpula lacrymans from spores and cord material brought back from the Narkanda region of the Himalayas...
N A White, J W Palfreyman, J Singh, S Singh


Water sprinkled pine wood: A microscope study on boards showing streaking
1993 - IRG/WP 93-10033
Boards sawn from the outer sapwood of pine lumber previously water sprinkled for periods of 10-18 weeks and kiln dried, showed streaking after staining. Two types of enhanced stain uptake were noted: 1) a more concentrated and localized form after 10 weeks and 2) a more diffuse type which developed in later stages of water sprinkling. Areas showing enhanced stain uptake were examined using light a...
G F Daniel, T Elowson, T Nilsson, A P Singh, K Liukko


Importance of bacteria in the deterioration of archaeological woods
1995 - IRG/WP 95-10122
An electron microscopic study of archaeological woods from different sites and of different ages revealed that the woods had been attacked by erosion bacteria, tunnelling bacteria and soft rot fungi. Bacterial erosion appeared to be most widespread, and was present independently as well as together with tunnelling and soft rot attacks. Thus, in many instances bacterial erosion was the only type of...
Yoon Soo Kim, A P Singh, T Nilsson


Ultrastructural aspects of bacterial attacks on an archaeological wood
1993 - IRG/WP 93-10007
Transmission electron microscopy of wood from a Chinese ship submerged in the mud for over 900 years showed bacteria to be the main factor for its deterioration. The micromorphology of degraded wood cell walls was similar to that observed during the attacks of wood by erosion bacteria. Other bacterial forms, previously considered lo be scavenging bacteria, were also abundant in degraded areas of t...
Yoon Soo Kim, A P Singh


The Relationship of Fiber Cell Wall Ultrastructure to Soft Rot Decay in Kempas (Koompassia malaccensis) Heartwoo
2004 - IRG/WP 04-10541
The ultrastructure of fiber walls in kempas (koompassia malaccensis) heartwood was examined in relation to soft rot cavity formation. The fibers consisted of middle lamella and thick secondary wall. The secondary wall was differentiated in to a S1 layer, and a unique multi-lamellar S2 layer. Two distinct forms of lamellae were recognisable, one type being considerably thicker than the other. They ...
A P Singh, A H H Wong, Yoon Soo Kim, Seung-Gon Wi


Ultrastructure of degraded, CCA-treated Pinus radiata wood from a marine pile
1990 - IRG/WP 1461
During an inspection of marine piles, 12 years after installation, severe degradation was noted on one of them in the vicinity of a corroded eye-bolt. The wood was dark brown in colour and tended to crumble easily. Wood fragments were examined by light microscopy and scanning and transmission electron microscopy and were also analysed for carbohydrates and lignin. Light microscopy showed numerous ...
A P Singh, M E Hedley


Novel observations on the micromorphology of soft rot attack of wood
1996 - IRG/WP 96-10176
Electron microscopic examinations of decaying Pinus radiata horticultural posts which had been treated with CCA preservative prior to being placed in service about nine years ago showed the presence of soft rot fungi and tunnelling bacteria. Some novel observations on the micromorphology of the soft-rotted areas of tracheid walls are described here. The micromorphology of soft-rotted areas was hig...
A P Singh, R N Wakeling


A study of decay type variability in variously treated Fagus sylvatica and Pinus radiata field test stakes exposed at a vineyard for 30 - 45 months
1998 - IRG/WP 98-10271
Pinus radiata test stakes were treated with 10 kg/m3 of CCA plus 4 lower retentions in a geometric series of 1.5. Fagus sylvatica was treated with 15 kg/m3 and 2 lower retentions. Both timber species were also treated with equivalent retentions of various new generation preservatives (P. radiata was also treated with creosote). Whilst these stakes were exposed at 11 sites in New Zealand (NZ) and 2...
R N Wakeling, A P Singh


Effect of mobile fungicides on fungal pre-infection in radiata pine billets.
1999 - IRG/WP 99-30191
In New Zealand, fungal infection of logs begins directly after felling and whilst severe degrade can be prevented if antisapstain-treated within 24 hours, such short turn-around times of logs are not always practicable. However, conventional antisapstain formulations in use only provide an envelope of protection on the log surface but are unable to control pre-infections below the surface. The obj...
D R Eden, T Singh, B Kreber


Natural Durability of Tropical Species – Variations and Prospects
2005 - IRG/WP 05-10568
The tropical timber resources of the world play an unequivocal role in economic development of both the tropical timber producing and importing regions. This paper describes natural durability as an important and preferred wood quality of tropical species of the world with emphasis on Malaysian hardwoods, the link between various aspects of tropical hardwood durability, hardwood utilization and bi...
A H H Wong, Yoon Soo Kim, A P Singh, Wang Choon Ling


Soft rot decay of Belian (Eusideroxylon zwageri) wood
1995 - IRG/WP 95-10119
The heartwood of Belian (Eusideroxylon zwageri), an East Malaysian timber species, is naturally durable due to its certain unique anatomical features and high content of extractives. The timber can tolerate years of exposure to hazardous conditions in ground contact and other situations without any significant loss in its strength. A few Belian transmission poles sampled from one locality in Saraw...
A H H Wong, A P Singh


A light and electron microscopic study of decayed CCA-treated radiata pine (Pinus radiata) wood from a cooling tower
1994 - IRG/WP 94-10056
An inspection of an industrial cooling tower in New Zealand showed surface decay of 12 year old Pinus radiata wood panels treated with CCA preservative to a retention of around 15 kg/m³ of salt. Wood decay micromorphology typical of that caused by soft rot fungi, white rot fungi, 'stripy' and 'v-shaped' erosion bacteria and cavitation bacteria were all commonly...
A P Singh, R N Wakeling, D R Page


Microbial decay of an archaeological wood
1994 - IRG/WP 94-10053
A light and transmission electron microscopic investigation of an archaeological wood was undertaken to determine the cause of its deterioration. The wood came from a bulwark constructed in early 1100 in the lake Tingstäde Träsk on the island Gotland in Sweden. The samples of the wood, which was identified as Pinus sylvestris, were taken from a depth of 0.85 m below the bottom level. The wood wa...
A P Singh, T Nilsson, G F Daniel


Microscopic characteristics of microbial attacks of CCA-treated radiata pine wood
1993 - IRG/WP 93-10011
Light and electron microscopic observations were made of CCA-treated radiata timbers, which had been placed in service in a vineyard soil as supporting poles and as part of a house pile, to determine the cause of their deterioration. The house pile had failed in service after between 9 and 13 years and was of particular interest because decay was more severe in deeper regions than at the surface a...
A P Singh, R N Wakeling


Biodegradation of wood in wet environments: A review
1997 - IRG/WP 97-10217
Wood in wet environments is attacked and degraded by soft rot fungi and erosion and tunnelling bacteria, which are more tolerant to high moisture and reduced oxygen conditions than basidiomycetes, such as white and brown rot fungi. Since basidiomycetes are normally more aggressive and can degrade wood faster than soft rot fungi and bacteria wood in wet environments can survive longer. In fact, arc...
A P Singh, Yoon Soo Kim


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