Your search resulted in 9 documents.
Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima
A case study of investigating fungi that affect traditional Japanese shake roof with/without copper plates
2013 - IRG/WP 13-10810
A Japanese traditional shake roof is made from split logs. To keep the roof long, copper (Cu) plates are often inserted between the shakes. It has been thought that Cu elements from copper plates would flow out when a shake roof is exposed to rain and inhibit growth of wood rotting fungi. Actually there are some examples where the Cu plates seem to be effective in preventing fungal damage, but on ...
T Wada, Y Fujiwara, Y Fujii, R Kigawa
Identification of soft-rot fungi existed in the samples from the galley excavated at Yenikapi
2014 - IRG/WP 14-10833
The shipwrecks of the Middle Byzantine period were excavated during the construction of the Marmaray railway and metro station in Yenikapı between 2004 and 2012. This has become the largest investigations because of the size and the number of the shipwrecks and its associated artifacts. In the previous report, the electron micrographs of the samples revealed that a wide range in the degree of...
T Wada, C Köse, K Igarashi
Corrosion verification experiment of the screw driven into CLT
2017 - IRG/WP 17-40816
Japan has abundant forest resources, but we depend on import for 70 percent of the consumed timber. Japan has the vision which promotes the timber self-sufficiency rate 30% to 50% by 2020. Therefore CLT attracts attention as a new timber material. Japan has a climate with high temperature and humidity, and wooden buildings are often treated with chemicals as a measure against biological deteriorat...
N Wada, H Ishiyama, S Yamamoto
Influence of sea water on timber and metal joints
2017 - IRG/WP 17-40818
In the Great East Japan Earthquake that occurred in March 2011, many buildings suffered from seawater immersion caused by the tsunami. Saito's study revealed that corrosion progresses on metal plate that experienced seawater immersion 1). However, the detailed mechanism of corrosion progresses has not yet been clarified. Therefore, in this study, salinity which is one of causes of corrosion w...
T Nishimura, H Ishiyama, F Hirose, N Wada
The Structural Performance of the Nail Driven into the Decayed Wood
2019 - IRG/WP 19-20652
In late years there are many actions to long-term excellent house realization. We have to consider repair or reinforcement for long-term and safety use of wooden house. There are various studies focused on structural performance of decayed wood. It is important for long-term use of wooden house that structural performance of nailed joint on decayed wood is accurately evaluated. However, there are ...
N Wada, H Isiyama
Influence of Drying Condition of Wood on Rusting of Nail
2019 - IRG/WP 19-40887
Recently in Japan, the durability of metal joint in wooden structures become important. Revised Building Standard Law was enacted in 2000 and Act on the Promotion of Popularization of Long-life Quality Housing was enacted in 2008 in Japan. Since then, the durability of the metal joints in the wooden houses becomes important. But there are not any detailed regulation for the durability of the metal...
H Ishiyama, N Wada, T Nishimura, T Mori
A novel cellulose-binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11019
Wood-rotting basidiomycetes are the major organisms decomposing wood in nature. They are classified into two groups based on their decay modes; white-rot fungi and brown-rot fungi. White-rot fungi secrete various cellulolytic enzymes during the wood degradation process. The enzymes are known to be often appended with a cellulose binding domain (CBD) which assists the activity of catalytic domain. ...
Y Kojima, N Sunagawa, M Aoki, M Wada, K Igarashi, M Yoshida
The cellulose binding mechanism of a novel cellulose binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11021
In nature, wood decay is caused by various wood-rotting basidiomycetes. Wood-rotting basidiomycete are mainly divided into white-rot fungi and brown-rot fungi. Their main carbon source is cellulose of the wood cell wall during wood decay, and they produce a variety of enzymes to decompose cellulose. The cellulolytic enzymes often possess a cellulose binding domain (CBD) as an additional domain con...
M Aoki, Y Kojima, M Wada, M Yoshida