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Natural durability transfer from sawmill residues of white cypress (Callitris glaucophylla). - Part 3: Full penetration of the refractory sapwood of white cypress
2000 - IRG/WP 00-40167
The heartwood of white cypress, Callitris glaucophylla, is renowned for its termite resistance and durability against decay. The sapwood, which can represent up to 30% of log volume, is non-durable and refractory to conventional preservative treatment. Previous work ascribes the lack of permeability to oily deposits within tracheids and ray cells. Environmental scanning electron microscopy was use...
M J Kennedy, L M Stephens, M A Powell


Resistance of Alstonia scholaris vestures to degradation by tunnelling bacteria
1992 - IRG/WP 92-1547
Electron microscopic examination of vessels and fibre-tracheids in the wood of Alstonia scholaris exposed to tunnelling bacteria (TB) in a liquid culture showed degradation of all areas of the secondary wall. The highly lignified middle lamella was also degraded in advanced stages of TB attack. However, vestured pit membranes and vestures appeared to be resistant to degradation by TB even when oth...
A P Singh, T Nilsson, G F Daniel


Soft-rot ultrastructure
1981 - IRG/WP 1138 (+ Addendum)
The stages of growth of soft-rot fungal hyphae in birch cell walls has been studied using transmission electron microscopy. These observations are compared with time-lapsed cinemicrographic studies on infection and cavity development within wood cell walls which show a start-stop hyphal growth pattern. The fine structure of hyphae during each stage of the decay process shows that hyphae penetratin...
M D C Hale, R A Eaton


Ultrastructural and TEM-EDAX studies on the degradation of CCA treated radiata pine by tunnelling bacteria
1985 - IRG/WP 1260
An ultrastructural study was carried out on bacterial attacked Pinus radiata stakes treated with a high level (24.7 kg/m³) of Tanalith NCA preservative. The purpose of the investigation was to determine whether the organism possessed intracellular and/or extracellular detoxification mechanisms in order to overcome the high levels of copper, chrome and arsenic present within the wood fibre walls. ...
G F Daniel, T Nilsson


Ultrastructure of the attack of a naturally durable timber by tunnelling bacteria
1990 - IRG/WP 1462
The attack of the wood of Eusideroxylon zwageri, a naturally durable species, by tunnelling bacteria (TB) was examined by light microscopy and transmission electron microscopy. Observations were made primarily on fibres. Parenchyma were included in some cases. Both fibres and parenchyma are rich in extractives. In fibres, extractives are primarily present in the lumen. The amber colouration of fib...
A P Singh, T Nilsson, G F Daniel


Water sprinkled pine wood: A microscope study on boards showing streaking
1993 - IRG/WP 93-10033
Boards sawn from the outer sapwood of pine lumber previously water sprinkled for periods of 10-18 weeks and kiln dried, showed streaking after staining. Two types of enhanced stain uptake were noted: 1) a more concentrated and localized form after 10 weeks and 2) a more diffuse type which developed in later stages of water sprinkling. Areas showing enhanced stain uptake were examined using light a...
G F Daniel, T Elowson, T Nilsson, A P Singh, K Liukko


Ultrastructural aspects of bacterial attacks on an archaeological wood
1993 - IRG/WP 93-10007
Transmission electron microscopy of wood from a Chinese ship submerged in the mud for over 900 years showed bacteria to be the main factor for its deterioration. The micromorphology of degraded wood cell walls was similar to that observed during the attacks of wood by erosion bacteria. Other bacterial forms, previously considered lo be scavenging bacteria, were also abundant in degraded areas of t...
Yoon Soo Kim, A P Singh


The Relationship of Fiber Cell Wall Ultrastructure to Soft Rot Decay in Kempas (Koompassia malaccensis) Heartwoo
2004 - IRG/WP 04-10541
The ultrastructure of fiber walls in kempas (koompassia malaccensis) heartwood was examined in relation to soft rot cavity formation. The fibers consisted of middle lamella and thick secondary wall. The secondary wall was differentiated in to a S1 layer, and a unique multi-lamellar S2 layer. Two distinct forms of lamellae were recognisable, one type being considerably thicker than the other. They ...
A P Singh, A H H Wong, Yoon Soo Kim, Seung-Gon Wi


Ultrastructure of degraded, CCA-treated Pinus radiata wood from a marine pile
1990 - IRG/WP 1461
During an inspection of marine piles, 12 years after installation, severe degradation was noted on one of them in the vicinity of a corroded eye-bolt. The wood was dark brown in colour and tended to crumble easily. Wood fragments were examined by light microscopy and scanning and transmission electron microscopy and were also analysed for carbohydrates and lignin. Light microscopy showed numerous ...
A P Singh, M E Hedley


Immunolocalization of extracellular metabolites from Tyromyces palustris
1991 - IRG/WP 1491
Polyclonal antisera produced to extracellular metabolites from the brown-rot fungus Tyromyces palustris was used in immunogold TEM studies. Gold labelling was detected in the fungal cell wall and extracellular slime layer but little or sporadic labelling was noted within the cytoplasm of the fungal hyphae. Gold particles were also found within the wood cell wall of Pinus densiflora decayed by Tyro...
Yoon Soo Kim


Formation of soft rot cavities in relation to concentric layers in wood fibre walls
1983 - IRG/WP 1185
A large number of timber species attacked by soft rot have been examined using light microscopy. The S2 layers in a large number of the timbers exhibited special structural features in the form of thin concentric layers. Several observations indicate that these layers may be characterised as "weak" zones by being more easily degradable than the surrounding wall layers. The chemical structure of th...
T Nilsson, G F Daniel


Soft rot decay of Belian (Eusideroxylon zwageri) wood
1995 - IRG/WP 95-10119
The heartwood of Belian (Eusideroxylon zwageri), an East Malaysian timber species, is naturally durable due to its certain unique anatomical features and high content of extractives. The timber can tolerate years of exposure to hazardous conditions in ground contact and other situations without any significant loss in its strength. A few Belian transmission poles sampled from one locality in Saraw...
A H H Wong, A P Singh


Biochemical aspects of white-rot and brown-rot decay
1987 - IRG/WP 1319
This paper presents an overview of the decomposition of wood by white- and brown-rot fungi - the most important and potent of known wood-decay fungi. These organisms are unique among cellulose destroyers because of their strong capability to enzymatically degrade lignified material. Special emphasis is given to the following aspects of wood decomposition by white- and brown-rot fungi: (1) effects...
T L Highley


Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh


Ultrastructure of Prumnopitys ferruginea wood from a buried forest in New Zealand
1991 - IRG/WP 1489
The buried wood of Prumnopitys ferruginea, a New Zealand native tree, was examined by scanning and transmission electron microscopy to investigate the extent to which the wood had deteriorated and the cause of its deterioration. Examinations showed that some deterioration of wood had occurred. Microscopy revealed a decay pattern similar to that produced by cavitation bacteria. Lenticular cavities ...
A P Singh, T Nilsson, G F Daniel


Effect of chitosan on the morphology and ultrastructure of two wood inhabiting fungi
2007 - IRG/WP 07-10606
An investigation was undertaken to compare the antifungal effects caused by chitosan against two selected wood degrading fungi Sphaeropsis sapinea and Trichoderma harzianum on their growth, morphological and ultra-structural characteristics, using differential interference microscopy, epifluorescence microscopy and transmission electron microscopy. The effect of chitosan on fungal morphology was ...
T Singh, A P Singh, G Daniel