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Decay resistance of Siberian larch wood against brown rot fungi - Part 2. The effect of genetic variation
1998 - IRG/WP 98-10287
Wood natural decay resistance is mainly restricted in the heartwood part of tree and based on wood microstructure and chemical composition. However, the variation of decay resistant in different wood species is vide and the effect of genotype on decay resistance of Siberian larch wood grown in Finland is not known. The aim of this work was to find out the variation on decay resistance of Siberian ...
H Viitanen, L Paajanen, T Nikkanen, P Velling


The critical moisture and temperature conditions for the growth of some mould fungi and the brown rot fungus Coniophora puteana on wood
1988 - IRG/WP 1369
The growth of some mould fungi on pine and spruce sapwood was studied in 40 different constant moisture and temperature conditions. The temperature range was between +10 and +40°C and the relative humidities varied from 76 to 100% relative humidity (RH). The incubation time was 12 weeks. The mixed mould inoculation used included typical fungi growing on wood: Aspergillus versicolor, Cladosporium ...
H Viitanen, L Paajanen


A light and electron microscopic study of decayed CCA-treated radiata pine (Pinus radiata) wood from a cooling tower
1994 - IRG/WP 94-10056
An inspection of an industrial cooling tower in New Zealand showed surface decay of 12 year old Pinus radiata wood panels treated with CCA preservative to a retention of around 15 kg/m³ of salt. Wood decay micromorphology typical of that caused by soft rot fungi, white rot fungi, 'stripy' and 'v-shaped' erosion bacteria and cavitation bacteria were all commonly...
A P Singh, R N Wakeling, D R Page


Microscopic characteristics of microbial attacks of CCA-treated radiata pine wood
1993 - IRG/WP 93-10011
Light and electron microscopic observations were made of CCA-treated radiata timbers, which had been placed in service in a vineyard soil as supporting poles and as part of a house pile, to determine the cause of their deterioration. The house pile had failed in service after between 9 and 13 years and was of particular interest because decay was more severe in deeper regions than at the surface a...
A P Singh, R N Wakeling


Biodegradation of wood in wet environments: A review
1997 - IRG/WP 97-10217
Wood in wet environments is attacked and degraded by soft rot fungi and erosion and tunnelling bacteria, which are more tolerant to high moisture and reduced oxygen conditions than basidiomycetes, such as white and brown rot fungi. Since basidiomycetes are normally more aggressive and can degrade wood faster than soft rot fungi and bacteria wood in wet environments can survive longer. In fact, arc...
A P Singh, Yoon Soo Kim


Statistical evaluation of 'micro-bending' samples for classification of wood attacking fungi
1992 - IRG/WP 92-1544
Micro-specimens from both hardwood and softwood were exposed to selected species of field isolated fungi. The strength reduction and weight loss caused by these fungi were determined. A statistical procedure was then developed to classify the fungi into brown-, white-rot, or non-decay fungal categories based on these data. The procedure reveals the extent of hazard a fungus poses to wood members. ...
B Goodell, Jing Liu, A Homola, J Jellison, J Shottafer


A study of the colonization of wood blocks in a laboratory unsterile soil test
1988 - IRG/WP 2318
CCA treated and untreated beech blocks were exposed to a defined horticultural loam using the method proposed for the collaborative soft rot test in the soft rot sub-group of Working Group Two. At intervals during the incubation wood samples were removed and fungal isolations were made using selective media. Fungi were identified and tested for their cellulolytic ability and their decay capacity i...
M T De Troya, S M Gray, D J Dickinson


Studies on the resistance of DMDHEU treated wood against white-rot and brown-rot fungi
2005 - IRG/WP 05-10566
Beech wood (Fagus sylvatica) modified with the textile finishing agent 1,3 dimethylol-4,5-dihydroxyethylen urea (DMDHEU) has shown to improve durability against the white-rot fungus Trametes versicolor. In a mini-block test, the weight loss over eight weeks of incubation decreased with increasing weight percent gain (WPG) of DMDHEU. At 25% WPG, no significant weight loss was observed, while untrea...
P Verma, C Mai, A Krause, H Militz


Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III


Extracellular hydrogen peroxide producing and hydrogen peroxide reducing compounds of wood decay fungi
1991 - IRG/WP 1516
Extracellular H2O2-producing and H2O2-reducing compounds were isolated from wood-containing cultures of all the white-rot and brown-rot fungi and Ascomycetes which well degraded wood, but were not detected in the culture of the fungi which degraded little wood. The compounds are glycopeptides with a low molecular weight, require H2O2 for one-electron oxidation, catalyze the redox reaction between ...
A Enoki, G Fuse, H Tanaka


Laboratory testing of wood natural durability - In soil-bed assays
1998 - IRG/WP 98-20141
Laboratory methods for assessing wood decay resistance are being investigated in the framework of an ongoing European research project. This paper summarizes the main results obtained for soil-bed tests based on the European prestandard ENV 807-Test 2. The data suggest that this testing methodology is suitable for evaluating the natural durability of timber species in soil-contact under conditions...
R Sierra-Alvarez, I Le Bayon, J K Carey, I Stephan, J Van Acker, M Grinda, G Kleist, H Militz, R-D Peek


Decay resistance of Platanus acerifolia wood against White Rot Fungi (Coriolus versicolor)
2002 - IRG/WP 02-10431
Degradation of the lignocellulose complex in wood varies on the microorganism causing decay and chemical composition of wood. The weight loss of Platanus acerifolia wood after decay by white rot fungus (Coriolus versicolor) for period 16 weeks were evaluated according to European standard EN 113 and beech wood as a control. The samples were collected in October 2000 from Nantes, France. The aim of...
M Irmouli, O Duchelier, N Ayadi, B Charrier


The biological effectiveness of wood modified with heptadecenylsuccinic anhydride against two brown rot fungi: Coniophora puteana and Gloeophyllum trabeum
1992 - IRG/WP 92-3705
A modified soil block test was carried out using wood samples reacted with heptadecenylsuccinic anhydride (ASA). This modification gave good resistance to decay brought about by the brown rot fungi Coniophora puteana and Gloeophyllum trabeum during the twelve week exposure period. Results indicated that there was a good correlation between increased loading of modifying reagent and an increase in ...
C Codd, W B Banks, J A Cornfield, G R Williams


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


Influence of acidification on decay processes of CCB treated wood
2004 - IRG/WP 04-10514
The phenomenon of copper tolerance by brown rot fungal strains has been known for a long period but the complete mechanism of copper tolerance by these fungi is not understood yet. Copper tolerance has previously been linked to oxalic acid excreted by copper tolerant brown rot fungal strains. This acid reacts with copper in the wood to form an insoluble and therefore less toxic copper oxalate. The...
M Humar, S A Amartey, M Šentjurc, F Pohleven


Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land


Synergistic combination of an antioxidant and wood preservative: a preliminary study
1998 - IRG/WP 98-30172
We previously proposed that extractives in highly durable angiosperm heartwood may protect wood against white-rot fungal colonization and subsequent degradation by a dual mechanism: extractives have some fungicidal activity and are also free radical scavengers (antioxidants) and thus interfere with the fungal free radical degradative mechanisms. We tested this hypothesis using the commercial bioci...
T Schultz, D D Nicholas, J Minn, K D McMurtrey, T H Fisher


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Natural Durability studies and Changes in wood chemistry of some Ghanaian hardwoods during decay by white- and brown-rot fungi
2004 - IRG/WP 04-10542
The natural decay resistance of Esa (Celtis mildbraedii), Wawabima (Sterculia rhinopetela) and Dahoma (Piptadeniastrum africanum) obtained from Ghana was determined according to BS EN 113 (1997). Esa was found not durable to the white-rot, but very durable to the brown-rot; Wawabima was very durable to both white- and brown-rots; and Dahoma was durable to the white-rot, and very durable to the bro...
Zeen Huang, K Maher, S A Amartey


The colonisation and succession of fungi in wood
1980 - IRG/WP 1107
A study of the microbial ecology of small stakes of Pinus sylvestris and Betula pendula, both untreated, and treated with a 1% solution of CCA is in progress. Results are presented for the early stages, up to 18 months.Two new techniques were developed linked by the philosophy of objective assessment. Isolations were made from the wood in a pre-determined pattern and transferred to four selective ...
C P Clubbe


Evaluating the performance of wood preservatives against fungi
1974 - IRG/WP 247
A compilation is made of results published by various workers for agar/block laboratory tests of the effectiveness of wood preservatives against 11 species of wood destroying Basidiomycetes and one species of soft rot fungus. Preservatives include creosote, boron, metal/arsenic mixtures, metallic naphthenates, chlorinated naphthalene, chlorinated phenols and tri-n-butyl tin oxide. The results are ...
R Cockcroft


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