Enzyme activities of Bursaphlenchus xylophilus and associated bacteria

Bursaphelenchus xylophilus or the pine wood nematode (PWN) is the causal agent of pine wilt disease, a destructive disease that affects coniferous masses. B. xylophilus causes severe economic, environmental, and social damage. The establishment of the disease depends on the ability of the PWN to degrade the lignocellulolytic material. Therefore, the study of enzymes able to hydrolyze these components is essential. The exact mechanism of invasion is still unknown and is assumed to be due to a complex interaction between the nematode, its vector, and its associated microorganisms. In order to provide new data on its pathogenesis, the secretions of the nematodes B. xylophilus and B. mucronatus plus a few of their associated bacteria was studied. Protease and CMCase activities of the secretions were evaluated.