The amount of spores produced by three strains of Trichoderma harzianum on the aerial mycelium of agar cultures and in shake cultures, respectively, correlated with the inhibition zones exerted against Phanerochaete chrysosporium in an agar diffusion test. The amount of soluble antifungal metabolites as well as the protein content also correlated with the inhibition zones and the amount of spores produced. The antifungal metabolites were identified to be trichorzianines. They were the only compounds with antifungal activity. It is concluded that the trichorzianines are responsible for the biocontrol effect by soluble metabolites and that they are produced during conidiogenesis.

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