The primary objective of the current investigation is to understand fungal interactions of dominant sapstaining fungi in radiata pine using confocal laser scanning microscopy (CLSM). In the current paper, an immunofluorescence labelling technique was developed for detection of Ophiostoma piceae using a monoclonal antibody. The primary antibody was labelled with Oregon green 514 Goat anti-mouse IgG fluorescent dye before examining with CLSM. The study showed that immunofluoresence labelling was achieved satisfactorily for detecting fungal hyphae of Ophiostoma piceae but not for some other important sapstaining fungi that occur in radiata pine.

Download document (1.3 Mb)
free for the members of IRG. Available if purchased.

Purchase this document