Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. Then, Fourier Transform Infrared analysis was carried out to monitor chemical changes of wood components. After extraction procedure, FTIR analysis was conducted again to obtain the spectra of extractives-free wood samples. IR spectra of mycelia were also recorded to identify bands other than wood components and avoid using them for calibration curves. The peak intensities of some lignin bands were correlated to lignin contents (Klason lignin plus acid-soluble lignin). This method is expected to be generally available for estimating lignin contents for brown-rotted woods decayed to different degrees.

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