The bamboo powderpost beetle Dinoderus minutus is a major pest of dry bamboo culms in Japan. In this study, acoustic emission (AE) and X-ray computed tomography (CT) was applied to the nondestructive analysis of the ovipositional behavior of adult D. minutus beetles inside bamboo pieces. Newly mated adult females were individually exposed to oviposition bamboo pieces prepared from culms of Phyllostachys bambusoides. AE measurement of the bamboo pieces were conducted continuously using AE sensors with a resonant frequency of 150 kHz. The bamboo pieces were scanned by the microfocus X-ray CT system with intervals of 2–5 d to observe the oviposition tunnels and eggs inside. Time courses of hourly AE hit rate showed that the female adults immediately started tunneling, and the tunneling activity continued almost ceaselessly. Oviposition tunnels bored across vascular bundles of bamboo and eggs deposited in the vessels were captured in X-ray CT images, and the length of oviposition tunnels and the number of eggs were measured.

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