Characterization of tropical wood-decaying fungi by RFLP analysis of PCR amplified rDNA

IRG/WP 98-10251

A Zaremski, M Ducousso, Y Prin, D Fouquet

Five brown-rot fungi (Tyromyces palustris, Gloeophyllum trabeum, Antrodia sp., Poria placenta, Coniophora puteana) and three white-rot fungi (Coriolus versicolor, Pycnoporus sanguineus, Lentinus squarrosulus) registred as representative wood-decaying fungi for international standards of wood durability tests have been characterized using molecular tools (PCR/RFLP). The Internal Transcribed Spacer between 18S and 25S rDNA has been amplified using ITS1 and ITS4 primers. The amplified fragments were digested using nine restriction enzymes (RFLP). The analysis of RFLP gel electrophoresis allowed to clearly discriminate every strain from each others. The 150 wood-decaying fungi strains currently in collection in the laboratory will be studied to create a data bank of reference profiles.


Keywords: PCR; RFLP; WOOD-DECAYING FUNGI; RDNA

Conference: 98-06-14/19 Maastricht, The Low Countries


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