Polyclonal antisera produced to extracellular metabolites from the brown-rot fungus Tyromyces palustris was used in immunogold TEM studies. Gold labelling was detected in the fungal cell wall and extracellular slime layer but little or sporadic labelling was noted within the cytoplasm of the fungal hyphae. Gold particles were also found within the wood cell wall of Pinus densiflora decayed by Tyromyces palustris. Erosion of wood cell wall and penetration of hyphae in the wood cell wall were frequently observed. The degradation pattern of lignocellulose by brown-rot fungus was also discussed.

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