Wood rotting basidiomycete is one of the major organisms that cause wood deterioration. Oxidoreductases secreted by those fungi are known to play key roles in the mechanism of wood cell wall degradation. As certain extracellular oxidoreductases including lignolytic peroxidases and lytic-polysaccharide monooxygenases from wood rotting fungi utilise hydrogen peroxide as a substrate in their catalytic reactions, the substance is thought to be a key factor in wood cell wall degradation by those fungi. To understand the wood cell wall degradation mechanism in wood rotting fungi, the information on enzymatic pathways for producing hydrogen peroxide is necessary. Among H2O2-producing enzymes, flavoenzymes belonging to auxiliary activity family 3 (AA3) in the database of carbohydrate active enzymes are widely conserved in fungi. However, the properties of most of AA3 enzymes from wood rotting basidiomycetes are unclear. In this study, novel AA3 enzymes from the wood rotting basidiomycetes Armillaria cepistipes are explored using protein structure prediction, and a selected AA3 enzyme from the fungus was attempted to be heterologously expressed for its characterisation.

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