The use of walnut extractives is interest as environment friendly wood preservatives in the wood preservation industry. In this study, organic solvents were used to remove extractives from Iranian walnut heartwood samples. The solvent was removed by rotoevaporation, and Identification of chemical compounds in the Walnut extractives was done by using GC/MS technique. The toxicity of various extracts concentrations against white rot was tested on agar media and the relative toxicity threshold determined at 0.15mg/ml of agar for Coriolus versicolor. Extracts were used to treat poplar blocks at three concentrations percent (1.5%, 2.5% and 3.5%). Treated blocks were exposed to white rot fungi to determine the biological performance of such treatment. Results showed that treated blocks weight loss to extracts retention of 1.99% in contrast with untreated poplar blocks against white rot, meanly decreased amounting to 15.65 percent, but for purposes of statistically have had not significant difference in 5 and 1 percent level. Also, resistant to tangential and radial hardness and resistant to compression parallel to grain of poplar blocks treated, for purposes of statistically have had not significant difference in 5 and 1 percent level. The most abundant identified compound in heartwood of walnut species was benzoic acid, 3,4,5-tris(hydroxy)/Gallic acid amounting to (44.57%) and the most toxic identified compounds, that caused decrease treated blocks weight loss, was Juglone (5.15%) and 2,7- dimethyle phenanthrene (5.81%).

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