Detection of a brown-rot fungus using serological assays

IRG/WP 1305

B Goodell, J Jellison

Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also used in an enzyme-linked immunosorbent assay (ELISA) to distinguish between Poria placenta and a non-Poria control fungus Rhizoctonia solani.


Keywords: PORIA PLACENTA; ELISA; SEROLOGY; FLUORESCENT ANTIBODIES; BROWN ROT; INCIPIENT DECAY; POLYCLONAL ANTISERA; DECAY DETECTION

Conference: 86-05-26/30 Avignon, France


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