Construction of an ITS sequence database for the identification and classification of wood rot fungi

IRG/WP 07-10626

S Horisawa, Y Honda, S Itakura, S Doi

Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic information of the wood rot fungi was analyzed for the construction of the database. The indoor fungi were selected from the fungal collection which is stored in the Deterioration Organisms Laboratory (DOL), The Research Institute for Sustainable Humanosphere (RISH), Kyoto University. Genome DNA was extracted from each isolate, and then the internal transcribed spacer (ITS) region was amplified by PCR using universal primer pair. The amplified DNA fragment was sequenced and was checked on the existing database such as GenBank in order to identify fungal species.

Keywords: wood rot fungi, ITS, rDNA, sequencing, database

Conference: 07-10-29/11-02 Taipei, Taiwan

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