The viable bacterial counts in sapwood and intermediate wood of sugi bolts increased immediately after floated in a fresh water pond. Bacterial invasion into the heartwood was not detected apparently during water-storage for 36 weeks, although a few bacterial species have inhabited in freshly-cut heartwood samples. The bacterial species isolated from the water-stored bolts were almost Gramnegative rods and scarcely isolated Bacillus spp. that has been thought to be one of the most effective species to change pit structures so far. Starch granules in parenchyma cells in sapwood and intermediate wood were disappeared after the water-storage for 4 weeks. After that degradation of bordered pit membranes were observed frequently in sapwood and partly in intermediate wood. The structures of cross-field pit membranes were changed in each position including heartwood.

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