Oxidative stress and lignin peroxidase production in Phanerochaete chrysosporium
M Morel, L Diss, C Fourrey, M Chalot, M Droux, J P Jacquot, E Gelhaye
In Phanerochaete chrysosporium liquid cultures, the induction of lignin peroxidases is directly related to accumulation of reactive oxygen species at the mitochondrial level. In this study, we demonstrate that the expression of the mitochondrial thiol-related antioxidant system is not directly coupled to the LiPs expression. When the antioxidant systems are not able to cope with ROS accumulation, a major change in the respiratory pathway leading to a large modification of the oxidative phosphorylation occurs. The adaptative system revealed by this study plays probably a central role in the LiPs expression regulation and could allow identifying new targets for wood preservatives.
Keywords: Phanerochaete chrysosporium, lignin peroxidase, alternative oxidase, alternative NADH dehydrogenase, mitochondria