A rapid colorimetric assay for mold spore germination using XTT tetrazolium salt

IRG/WP 11-20462

C A Clausen, V W Yang

A rapid colorimetric assay was developed to quantitate metabolic activity in mold spores during germination using 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[9phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT). The assay was used to demonstrate inhibition of spore germination following exposure to different biocides and variability in the inhibition of spore germination of different mold species to the same biocide.

Keywords: tetrazolium salt, colorimetric, assay, mold, spore germination

Conference: 11-05-08/12 Queenstown, New Zealand

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