A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA were reached to 50 ng/μl, which was approximately 1,000 times higher than that of DNA in the original sample. It was revealed that the increase of PCR cycles biased basidiomycete species from original ratio, whereas longer incubation time of the non-specific amplification less affected on it, suggesting the advantage of amplification by Phi29 DNA polymerase to analysis on dynamics of basidiomycetes in decayed wood.

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