The copper tolerance of two brown rot fungi, Gloeophyllum sepiarium and Oligoporus placentus, has been tested using both mycelia and basidiospores as inoculum sources. Mycelial growth of O. placentus on agar medium was shown to be much more tolerant to copper (as copper sulfate) than that of G. sepiarium. However, there was no difference in copper tolerance of basidiospores between the two species. Spores were much more sensitive to copper than mycelia in both species. This may explain why copper-based preservatives without co-biocide perform well in stake tests unless there is copper tolerant fungi mycelium in the soil.

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