The effect of storage and subculturing on in vitro fruit body formation and spore production in Gloeophyllum sepiarium and Oligoporus placentus
S Choi, J N R Ruddick, P I Morris
Spores are widely assumed to be the main mode of infection of wood in conditions conducive to decay above ground. In order to supply spores on demand as an experimental material, fruiting body and basidiospore production in vitro by Gloeophyllum sepiarium and Oligoporus placentus have been examined. The optimum medium, time to spore production, and duration of fruiting as well as fruiting body shape varied among strains of both species. Even when the same strain was used as the inoculum source, there were differences in duration of fruiting and time to spore production in different experimental sets. This suggests that the method of mycelium storage and repeated subculturing could affect fungal capacity for fruiting and basidiospore production. Among the tested methods of mycelium storage, mycelia stored on wood blocks showed the most stable response in both fruiting and spore production.