Juglone was extracted from Juglans mandshurica bark via vacuum-assisted steam stripping. The extract was characterized by Fourier Transform Infrared Spectroscopy (FTIR), 1H Nuclear Magnetic Resonance (NMR) and Gas chromatography/mass spectrometry (GC-MS). The extraction process was easy to perform and produced excellent yields (0.22 g juglone/100 g of bark) of a high purity extract (96.42% juglone). The yield was much higher than those of previous studies. The biological activity of the extract was assessed in vitro against Aspergillus niger, Paecilomyces variotii, Trametes versicolor and Gloeophyllum trabeum. Juglone showed good anti-fungal activity, even at concentrations as low as 500 ppm. The results suggest that Juglone may show promise as a natural wood protectant and further studies are planned.

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