Immunolocalization of extracellular metabolites from Poria placenta

IRG/WP 1361

B Goodell, G F Daniel, J Jellison, T Nilsson

Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected in the extracellular slime layer surrounding hyphae. Labelling occurred within the wood cell wall, but little non-specific labelling was noted within the cytoplasm of the fungal hyphae. A vesicle-like body within the hyphae did exhibit specific labelling.


Keywords: DEGRADATION; BROWN ROT; PORIA PLACENTA; IMMUNOFLUORESCENCE MICROSCOPY; TEM; IMMUNOLABELLING; EXTRACELLULAR METABOLITES; DECAY; CELL LOCALIZATION

Conference: 88-04-24/29 Madrid, Spain


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