Attempts to control and optimize the production of hymenial structures and basidiospore production in Gloeophyllum sepiarium in axenic culture resulted in the proposal of the following conditions as being suitable. The dikaryotic mycelia originally isolated from basidiocarps could consistently be induced to produce hymenial structures and pure basidiospore collects if illuminated by near ultraviolet light with emission maximum at 355 nm ("black light") at a temperature of 15°C on a chemically defined medium, where the concentration of the carbon and the nitrogen sources were shown to be of critical significance. The necessary conditions for basidiospore production in lignicolous fungi in general are is briefly discussed.

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