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A bioassay to determine preservative retention in hardwoods and southern pines
1982 - IRG/WP 2183
In this paper the author discusses and suggests a new method of evaluating preservative retention in treated woods by means of bioassays. Twelve North American species were tested with different retention levels of pentachlorophenol (PCP) and copper/chrome/arsenic (CCA) preservatives. The results observed in this work show that among other fungi, Aspergillus niger responds to both preservatives, w...
J C Moreschi


International collaborative laboratory comparison of two wood preservatives against subterranean termites: Second update
1995 - IRG/WP 95-10117
As was agreed by members at the termite workshop at the 24th annual meeting of IRG in Orlando, USA, in May 1993 to initiate an international subterranean termite laboratory bioassay to compare the various preferred termite protocols used by IRG termitologists was initiated. The author was nominated to co-ordinate this comparative laboratory evaluation of two wood preservatives (CCA and Cu-naphthen...
J R J French


A study on the effectiveness of didecyldimethylammonium chloride to protect wood from attack by termites
1993 - IRG/WP 93-30009
A laboratory bioassay was conducted on the ability of didecyldimethylammonium chloride (DDAC) to protect Pinus radiata D.Don wood specimens from attack by two of Australia's most economically important species of subterranean termite, Mastotermes darwiniensis Froggatt and Coptotermes acinaciformis (Froggatt). Sapwood specimens of Pinus radiata, treated to achieve nominal retentions of 0.5...
J W Creffield


A laboratory bioassay method for testing preservatives against the marine borers Limnoria tripunctata, L. quadripunctata (Crustacea) and Lyrodus pedicellatus (Mollusca)
1990 - IRG/WP 4159
A laboratory culture and bioassay method is described for the marine borers Limnoria tripunctata, Limnoria quadripunctata, and Lyrodus pedicellatus. The methods were tested in a bioassay using established marine preservatives. The attack produced on blocks treated with CCA or creosote in some ways paralleled the attack found in the sea. Limnoria tripunctata attacked treated and untreated pine bloc...
L J Cookson


Determination of fungitoxic value of preservatives in laboratory wood-block tests. Part 2: Statistical estimation
1989 - IRG/WP 2327
Laboratory modified agar-block tests were made to determine the fungitoxic value of the wood preservative CCA against the test fungus Serpula lacrymans using statistical methods. With the aid of statistical computer programmes based on the least square method. the regression equation was found for the dependence between sample mass loss and preservative retention both in terms of percentage and fo...
J Wazny, J D Thornton, K J Krajewski


Determination of fungitoxic value of preservatives in laboratory wood-block tests. Part 1: Standard procedures
1989 - IRG/WP 2326
By applying a modified agar-block method, a comparative analysis was made on the toxic value of the wood preservative CCA against the test fungus Serpula lacrymans. The procedure applied to determine the results accounted for different standards: EN (Toxic limit), ASTM (threshold retention), GOST (threshold retention and protection probability), PN (toxic doses) and JIS (value of efficiency). Desp...
J Wazny, J D Thornton


Specifying preservative-treated resistant timber: Conforming to European Standards
2001 - IRG/WP 01-20235
The most common construction timbers used in the UK are of low natural durability and, generally, resistant to preservative treatment. These include species of spruce, hemlock and fir. However, their characteristically limited and non-uniform uptake of preservatives may still confer sufficient protection to give satisfactory durability performance in terms of biological resistance. This paper desc...
E D Suttie, A F Bravery, T B Dearling


Inter-laboratory comparison of assessment methods for wood preservatives against subterranean termites: Determination of protection threshold limits for CCA
1999 - IRG/WP 99-10317
In 1996, several institutes conducted laboratory bioassays on the efficacy of unleached Pinus radiata specimens treated with copper-chromium-arsenic (CCA) and copper naphthenate (Cu Naph) at retentions of 0.5, 1.0, 2.0 and 4.0 kg/m3 (total salt for CCA; elemental copper for Cu Naph) against a range of species of subterranean termites (IRG/WP/96-10174). Each participant employed the standard test m...
J W Creffield, M Lenz


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


Determination of toxicity data for preservatives against Basidiomycetes by measuring the reduction in compression strength of wood
1988 - IRG/WP 2297
Blocks of Pinus radiata D. Don were treated to 12 retention levels with CCA and NaPCP. These were exposed for 12 weeks in agar jars to the dry rot (brown rot) Serpula lacrymans (Schum. ex Fr.) S.F. Gray. After mass loss determination, the same blocks were tested for reduction in compression strength parallel to the grain. Using both these parameters, together with interpolated 6 week strength loss...
J Wazny, J D Thornton


A bioassay of remedially treated pole material
1987 - IRG/WP 3453
Stubs prepared from treated and untreated utility poles were installed in a test plot and pressure spade treated at the groundline with one of four preservative greases. At one and three year intervals, core samples were removed from the stubs and the improvement in the preservative efficacy due to the greases was assessed. The bioassay investigation showed that only bis(tributyltin) oxide (TBTO) ...
J N R Ruddick, L Parker


A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton


Boracol 40 - A potential remedial and preservative treatment for lyctids
1983 - IRG/WP 1192
The paper reports on a preliminary assessment of the efficacy of a diffusion formulation, Boracol 40, when applied as a brush treatment: to the surface of Eucalyptus obliqua against the powder-post borer Lyctus brunneus. After a five month bioassay, Boracol 40 has severely retarded the progress of active infestations of Lyctus brunneus and has been 100 per cent effective as a preventative treatmen...
J W Creffield, H Greaves, C D Howick


Laboratory bioassay and field trial on imidacloprid and cypermethrin as glueline treatments for softwood plywood
2006 - IRG/WP 06-30405
The effectiveness of imidacloprid and cypermethrin as glueline treatments for protecting phenol formaldehyde (PF)-bonded Pinus radiata (radiata pine) plywood from attack by subterranean termites was evaluated both in the laboratory and field. Imidacloprid was evaluated in two plywood constructions (19 ply x 1.6 mm-thick veneers and 5 ply x 3.2 mm-thick veneers) whereas cypermethrin was evaluated i...
J W Creffield, D K Scown


Methods for Determining the Role of Extractives in the Natural Durability of Western Redcedar Heartwood
2007 - IRG/WP 07-20356
The durability of western redcedar heartwood is influenced by a number of different extractives operating by different mechanisms. In order to guide a systematic isolation of extractives with high fungal toxicity, a micro-bioassay has been developed for measuring the fungal toxicity of extractive fractions. Two additional assays quantify the anti-radical and metal chelating properties of cedar ext...
R Stirling, C R Daniels, J E Clark, P I Morris


Detection of Anti-Fungal Sapwood Extractives in Non-Durable Scots Pine (Pinus sylvestris), Rubberwood (Hevea brasiliensis) and Jelutong (Dyera costulata)
2007 - IRG/WP 07-10634
A general laboratory bioassay method of Woodward and Pearce (1985) was adopted to detect anti-fungal activity of sapwood or heartwood extractives of 5 Malaysian hardwoods [dark red meranti heartwood (Shorea spp.), red balau heartwood (Shorea spp.), kulim heartwood (Scorodocarpus borneensis), jelutong sapwood (Dyera costulata) and rubberwood sapwood (Hevea brasiliensis), including the temperate Pin...
A H H Wong, R B Pearce


Performance of sintered glass screening as a potential physical barrier against subterranean termites in the laboratory and after 4 years of field test
2008 - IRG/WP 08-10646
This paper describes the performance of sintered glass screenings as a potential physical barrier against the subterranean termites, Coptotermes acinaciformis and Mastotermes darwiniensis in the laboratory and after four years of field testing in active above-ground mound colonies of Coptotermes lacteus. The laboratory results suggest that sintered glass is a viable control option against Copto...
J R J French, B M Ahmed (Shiday)


Laboratory study on the termiticidal efficacy of Eremophilone oil
2009 - IRG/WP 09-30497
A novel timber preservative formulation, Termilone® TT, is being developed by BioProspect Limited. This environmentally friendly preservative formulation incorporates Eremophilone oil, extracted from Eremophila mitchellii, as the active ingredient. Chemical analysis performed on Pinus radiata sapwood specimens treated with the Termilone® TT formulation revealed that the Eremophilone oil is relat...
D K Scown, J W Creffield, R Spooner-Hart


Laboratory and field evaluation of Laminated Strand Lumber treated with Borogard® ZB against termites
2009 - IRG/WP 09-30498
A laboratory bioassay and subsequent field trial was undertaken to determine the termiticidal effectiveness of a Laminated Strand Lumber (LSL) composite when treated with Borogard® ZB. The bioassay and field trial were conducted against two of Australia’s most economically important species of termite, Mastotermes darwiniensis and Coptotermes acinaciformis. Against both species of termite, LSL ...
D K Scown and J W Creffield


Design, synthesis, characterisation and effectiveness of ‘Locked-in-Boron’ chemicals for H3.2 level of wood protection
2011 - IRG/WP 11-30577
Boratrane molecules with five-membered and six-membered molecular ring systems, and with various ring substituents were synthesised, characterised and formulated for wood treatment for accelerated laboratory bioassays using wood decay fungi as test organisms. Six-membered ring boratranes showed lower efficacy in laboratory assays than five-membered ring boratranes. One alkyl-substituted boratran...
R Franich, H Kroese, S Gallagher, S Hill, B Kelly, G Billett, R Meder, W Rae


Preliminary laboratory bioassay to evaluate engineered Fibre-boards against subterranean termites C. acinaciformis (Isoptera: Rhinotermitidae)
2012 - IRG/WP 12-10768
In Australia, laboratory bioassays with subterranean termites typically contain groups of workers and soldiers in a substrate of moist mound material. This termite substrate may affect termite consumption, and the test materials create a favorable termite condition for the test. The main purpose of this test is to examine the effect of a melamine formaldehyde treatment of wood panels to evaluate t...
B M Ahmed (Shiday), H Militz, B Ozarska, I Ali, L Kloeser


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