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The proposal for optimalization of the agar-block method for wood preservatives fungitoxic evaluation
1995 - IRG/WP 95-20065
On the base of own research and other scientists results the proposal for optimalization of agar-block method was presented by: - selection of test fungal species and strains and central distribution of their pure cultures, - change of the treatment and control samples exposure procedure, - application of mathematical estimation of toxic value results, - shortening the duration of fungitoxic t...
J Wazny


The use of plastic meshes in soft rot monoculture testing
1990 - IRG/WP 2353
Plastic meshes were introduced between the wood blocks and agar medium in a miniaturised soft rot monoculture test in order to minimise transfer of the preservative from the wood and mineral salts from the agar. Although several different sizes and types of mesh were used and the blocks were wetted up to an appropriate moisture content for soft rot attack the amount of decay was substantially redu...
S M Gray


A novel defaunation method of the protozoa to investigate cellulose metabolism in Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)
1994 - IRG/WP 94-10050
The largest protozoa in the hindgut of workers of Formosan subterranean termite, Coptotermes formosanus Shiraki, was selectively eliminated by forced-feeding on low-molecular weight cellulose (LC) with a mean DP of 17. Although one week's feeding on LC caused perfect disappearance of Pseudotrichonympha grassii Koidzumi, the selective defaunation method itself had no detrimental effect on ...
T Yoshimura, K Tsunoda, M Takahashi


Immunogold localisation of catalytic domain of endocellulase in the cellulosic substrates
1997 - IRG/WP 97-10205
The reaction of catalytic domain of endocellulase on the cellulosic substrates was visualised by using immunogold labelling procedures to obtain a better understanding of the mechanism of enzymatic cellulose hydrolysis. Polyclonal antibody was produced against the catalytic domain of endo-l, 4-ß-glucanase (EGI) from a ruminal anaerobic bacterium Ruminococcus albus F-40. Immuno-TEM works showed th...
Yoon Soo Kim, Seung-Gon Wi, Kyu Ho Myung, K Ohmiya, S Karita


Ultrastructure of degraded, CCA-treated Pinus radiata wood from a marine pile
1990 - IRG/WP 1461
During an inspection of marine piles, 12 years after installation, severe degradation was noted on one of them in the vicinity of a corroded eye-bolt. The wood was dark brown in colour and tended to crumble easily. Wood fragments were examined by light microscopy and scanning and transmission electron microscopy and were also analysed for carbohydrates and lignin. Light microscopy showed numerous ...
A P Singh, M E Hedley


Defining fungal decay types - Final proposal
1988 - IRG/WP 1355
The term soft rot is proposed for all forms of decay caused by Ascomycetes and non-basidiomyceteous Fungi imperfecti. The terms brown rot and white rot should be used only for decay caused by Basidiomycetes. Brown rot is characterized by extensive depolymerization of the cellulose and limited lignin degradation. White rot is characterised by significant degradation of the lignin component in wood....
T Nilsson


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


The effect of malt and agar trademarks on growth, decay and stilbene resistance of fungi
2000 - IRG/WP 00-20202
Effects of malt and agar trademarks used in growth media on growth of fungi in cultures, amount of decay they cause in birch samples and their resistance to stilbenes were tested. It was tested also had the conditions where fungus cultures were stored before inoculation of test plates some effect on the results. Fungi used in these tests were white rot fungus Trametes versicolor and brown rot fung...
L Syrjälä, L Paajanen, A Pappinen


Comparison between agar-block and soil-block methods for wood-destroying Basidiomycetes
1992 - IRG/WP 92-2401
The object of this work is to compare these methods in order to provide some information to help in the choice between them. The comparison was made by a statistical analysis (factorial completely randomized design) and by a discussion about other aspects of each method. An evaluation of some fungi on attacking wood and a comparison between Picnoporus sanguineus isolated from carpophore and decaye...
M B B Monteiro, S Brazolin, G Catanozi


An electron spin resonance study of manganese changes in wood decayed by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1359
Electron spin resonance (ESR) spectrometry was used to examine wood decay by the brown-rot fungus, Postia placenta. Wood slivers of Douglas-fir, white fir, redwood, sweetgum and yellow poplar were incubated for 4 weeks in custom-made quartz ESR tubes with or without Postia placenta. In all wood species without fungus, a weak partially resolved signal (about g=2, presumably due to manganese) was de...
B Illman, D C Meinholtz, T L Highley


Assessment of the toxicity of some copper-, zinc- and boron-based wood preservatives to the cellar fungus Coniophora cerebella Schröet
1974 - IRG/WP 242
This article reports the use of a method based on the determination of the probability of the protection of timber against destruction by fungi. By converting the probability values to probit values and plotting them as a function of the amount of preservative retained in the timber, curves of the toxic effect are obtained, enabling any timber protection probability to be assessed....
V N Sozonova, D A Belenkov


Natural weathering of wood in a sunny climate effects on surface chemistry and paint adhesion
1997 - IRG/WP 97-20109
Radiata pine veneers and blocks were exposed to natural weathering under Australian summer conditions over a period of 30 days. Infrared spectroscopy revealed that there was perceptible surface delignification after 4 hours exposure, substantial surface delignification after 3 days exposure and almost complete surface delignification after 6 days. Viscometry determinations on holocellulose samples...
P D Evans, P D Thay, K J Schmalzl


Fungi used in standard tests on the toxicity value of wood preservatives in various European countries
1975 - IRG/WP 255
The aim of the present paper is to make the comparative analysis of test fungi used in various European countries in order to define the toxicity value of wood preservatives against fungi of the Basidiomycetes class. Only the methods with national standard rank, present on the currently binding standards list are taken for consideration. The analysis of similarities and differences in the choice o...
J Wazny


Collaborative soft rot tests: Paper for discussion in Working Group II
1970 - IRG/WP II 5A
An account of the major part of the collaborative work on laboratory tests of toxicity of preservatives to soft rot fungi initiated by the European Homologation Committee at Delft in 1966 has been published in ‘Material und Organismen’ (1970) as a report of progress. The main objective of these collaborative tests, namely to establish a reliable and acceptable standard test method was not acco...
J G Savory, A F Bravery


Some data on the activity of alternative fungicides for wood preservation
1985 - IRG/WP 3333
Data from laboratory tests against basidiomycete fungi are presented for 9 alternative fungicides in organic solvent formulations and also in water for one product. Results are compared with data for reference preservatives, tributyltin oxide, copper and zinc naphthenates and pentachlorophenol. Of special interest is the apparently better than additive effect of mixing tributyltin naphthenate and ...
A F Bravery, J K Carey


Effect of angle of exposure on degradation of radiata pine during weathering trials
1988 - IRG/WP 2301
The importance of angle of exposure on the degree of weathering of materials has not been adequately resolved. In conventional tests, specimens are exposed at an angle of 45° facing the equator, but more recently experiments on polymeric materials have suggested that an angle of 0° may be preferable since this angle maximises levels of received ultra-violet radiation. In this paper the degradati...
P D Evans


Preliminary results of the treatment of wood with chlorosilanes
1981 - IRG/WP 3172
It is clear from the initial data reported here that the treatment of pine sapwood with chlorosilanes under the reaction conditions employed did not significantly reduce the decay by both white rot and brown rot fungi. Only the dichlorosilane compounds showed to possess some protective action against fungal attack. Before drawing conclusions on the application of organosilicon compounds as potenti...
M Stevens


Hygroscopicity of decayed wood - Implications for weight loss determinations
1996 - IRG/WP 96-20085
Hygroscopicity changes were observed in red maple blocks subjected to brown rot, white rot and soft rot. Brown rot reduced hygroscopicity, soft rot increased hygroscopicity, and white rot showed only a very slight increase in hygroscopicity. The effect of these changes on weight loss tests is a slight overestimation of weight loss for brown rot and a slight underestimation for soft rot and white r...
S E Anagnost, W B Smith


Soft rot decay in acetylated wood. Chemical and anatomical changes in decayed wood
2002 - IRG/WP 02-40231
Acetylated Beech (Fagus sylvatica) and Scots Pine (Pinus sylvestris) wood were studied in soil beds under laboratory conditions for longer periods. High mass losses and dynamic MOE losses were measured in non-acetylated wood and at lower weight percent gains (WPG). Rapid losses of lignin, holocellulose and ?-cellulose occurred in non-acetylated beech wood and no losses were detected at the highest...
B Mohebby, H Militz


Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen


Effects of alkali treatment on some mechanical and chemical properties of creosote treated oaks
1991 - IRG/WP 2366
To date, there is a lack of information on the effects of chemical treatment on the performance of creosote preservative treated oak sleepers. This factorial experiment was designed to analyze three main effects: species (Quercus alba and Quercus rubra) creosote treatment (treated and untreated), and alkali (NaOH) soaking (0, 1, and 10 percent). The modulus of elasticity (MOE) and fiber stress at ...
P Chow, A J Reinschmidt, E J Barenberg, L C Chang


Biochemical relationships between biodegradation of cellulose and formation of oxalic acid in brown-rot wood decay
1991 - IRG/WP 1472
Non-enzymic hydrolysis of cellulose with low concentrations of oxalic acid was examined. The incubation of pine wood pulp with 1% oxalic acid (pH 1.3) at 35°C for 4 weeks reduced the original viscosity to 60%. Reducing sugars were liberated from various cellulosic samples by the oxalic acid treatment. However, crystallinities of cellulose in those samples did not change before and after the treat...
M Shimada, Y Akamatsu, A Ohta, M Takahashi


Biocidal screening method of wood extractives by a direct use of cellulose TLC plate
2001 - IRG/WP 01-20226
Most of the methods to evaluate the biological durability of woods have focused on decay fungi and termite as deteriorating organisms and it is well known that one of the most important factors affecting the biological characteristics of wood is extractives. Bioassays for evaluating the biological activities of wood extractives have been mainly conducted with treated filter papers for termites or ...
R Yusiasih, T Yoshimura, T Umezawa, Y Imamura


Protection of wood blocks treated with Trichoderma isolates selected on the basis of preliminary agar screening studies
1996 - IRG/WP 96-10154
Previously reported results of agar interaction screening studies for biocontrol agents of wood decay basidiomycetes showed two Trichoderma viride isolates, killed 16 of 19 target fungi (Tucker and Bruce, 1995). Testing of these isolates in wood was required to assess their performance at preventing decay of wood blocks. Standard testing of chemical wood preservatives is used to determine the toxi...
E J B Tucker, A Bruce, H J Staines


Soft-rot control in hardwoods treated with chromated copper arsenate preservatives. Part 3: Influence of wood substrate and copper loadings
1977 - IRG/WP 2100
The hypothesis is proposed that hardwoods need more chromated copper arsenate (CCA) than softwoods to protect them from soft-rot attack mainly because hardwoods are more readily consumed by soft-rot fungi. Simple model systems, using copper-supplemented agar or groundwood pulp treated with CCA showed that fungi tolerated more toxicant (copper) as more available substrate (malt) was provided. Soft-...
M A Hulme, J A Butcher


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