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Biocidal screening method of wood extractives by a direct use of cellulose TLC plate
2001 - IRG/WP 01-20226
Most of the methods to evaluate the biological durability of woods have focused on decay fungi and termite as deteriorating organisms and it is well known that one of the most important factors affecting the biological characteristics of wood is extractives. Bioassays for evaluating the biological activities of wood extractives have been mainly conducted with treated filter papers for termites or ...
R Yusiasih, T Yoshimura, T Umezawa, Y Imamura


Some recent studies on the marine wood-borers of the west coast of India
1982 - IRG/WP 486
Occurrence and distribution of marine wood-borers along the west coast of India from Mangalore to Kandla are presented together with the distribution pattern of all the molluscan and crustacean wood-borers so far reported from India coasts. The survey, conducted for the first time along this coast, revealed the presence of 14 species of Teredinidae, 2 species of Pholadidae, 3 species and one varie...
L N Santhakumaran


Quantification of wood decay effects by HPLC analysis
1992 - IRG/WP 92-1576
The present work quantified the effects of the white rot basidiomycetes Coriolus versicolor and Phanaerochaete chrysosporium, and also those of the brown rot fungi Coniophora puteana and Lentinus lepideus, on Pinus patula and Eucalyptus grandis. Wood colonisation was quantified by Kjeldahl nitrogen determinations converted to biomass assays, and degradation was quantified by weight losses produced...
V Singh, M Tarin, G D Shelver, A A W Baecker


Distribution of the three symbiotic protozoa in Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)
1993 - IRG/WP 93-10010
Six colonies (three each from laboratory and field) of Formosan subterranean termite, Coptotermes formosanus Shiraki, were served for investigating the abundance and distribution of three symbiotic protozoa in the hindgut of workers. The total protozoan number amounted to 6,000-10,000 per a worker, and the order of the abundance of the three protozoa and the proportional distribution of each speci...
T Yoshimura, K Tsunoda, M Takahashi


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


The role of oxidation in wood degradation by brown-rot fung
1992 - IRG/WP 92-1562
Brown-rot fungi are suggested to degrade cellulose by oxidation with hydrogen radicalsformed eg. in the conversion of hydrogen peroxide in the Fenton type reactions. The stuctural changes in the holocellulose in wood induced by Fenton's reaction on wood components are very similar to those caused by brown-rot fungi. In this work the effect of the Fenton reaction on wood components was stu...
A-C Ritschkoff, J Pere, J Buchert, L Viikari


Assessing the importance of degradation mechanisms on the loss of effectiveness of wood preservatives
2000 - IRG/WP 00-20193
Accelerated ageing systems developed for application to samples in the laboratory prior to biological tests, should reflect those natural deterioration processes that are likely to occur in the hazard classes defined in EN 335-1. Losses through evaporation or the effects of leaching have been recognised, however their importance, relative to other mechanisms has not been quantified. Degradation me...
E D Suttie, R J Orsler, T Dearling


Properties of cellulose degraded by the brown rot fungus, Postia placenta
1988 - IRG/WP 1350
To gain further understanding of the nature of the cellulose depolymerizing agent or agents of brown-rot fungi, brown-rotted cellulose was chemically and physically characterized. Various culture conditions, such as low nitrogen and elevated oxygen levels, did not induce degradation of cellulose by Postia placenta in liquid cultures. Therefore, brown-rotted cellulose for analysis was prepared by a...
T L Highley, R E Ibach, T K Kirk


Breakdown of cellulose derivatives by cellulolytic enzymes. I. Action of fungal ß-glucosidases toward substituted PNP ß-D-glucopyranosides
1998 - IRG/WP 98-10281
The action of fungal ß-glucosidases on the methyl derivatives of p-nitrophenyl (PNP) ß-D-glucopyranoside, which were regioselectively substituted at 0-2, 0-3, 0-4 and 0-6 positions was studied. Several ß-glucosidases from brown-rot, white-rot, and soft-rot fungi and almond were used for the study. These ß-glucosidases did not act on the 2, 3, and 4-0-methyl derivatives, while the 6-0-methyl on...
T Nishimura, I Momohara, M Ishihara


Fruitbody formation and basidiosporogenesis by the white rot fungus Pycnoporus cinnabarinus
1988 - IRG/WP 1348
Conditions for fruitbody formation by the white rot fungus Pycnoporus cinnabarinus in vitro were outlined. A marked difference in substrate requirements in comparison to previously tested brown rot fungi was revealed. Agar media containing Walseth cellulose and NH4 tartrate at 1 g/l permit profound production of basidiospores particularly at 15°C but only under light treatment. Higher NH4 tartrat...
J Bjurman


Weathering of copper-amine treated wood
2000 - IRG/WP 00-40155
Copper ethanolamine (Cu-EA) treated southern pine (SP) were artificially weathered with a QUV Weathering Tester. The weathering regime was composed of a continuous UV-light irradiation for 2 hours followed by a water spray for 18 minutes for a total time of 1200 hours. The changes on the surface of the weathered samples were characterized by Fourier transform infrared spectroscopy (FTIR) analysis,...
J Zhang, D P Kamdem


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Comments on leaching in view of accelerated testing
1977 - IRG/WP 294
We developed an accelerated test for leaching at elevated temperatures (75-85°C). This test was used for the examination of 11 preservative formulations containing Cu, Zn and As. The data obtained from these experiments were compared with data on some formulations tested by ASTM standard leaching test. The method consists in making a large surface to volume ratio of wood from a treated block by c...
J Rak


Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early stages of decay, osmiophilic particles were found with all the fungi investigated. In the brown-rotted wood, the osmiophilic particles occur...
K Messner, H Stachelberger


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


Decomposition of biocides in aqueous media cases of MTC and TCMTB
1993 - IRG/WP 93-50001-26
Our laboratory is interested in the fate of modern biocide molecules in the environment. Here we present recent work which has been done on the fate of two compounds with fungicidal and bactericidal properties: methylenedithiocyanate (MTC: NCS - CH2 - SCN) and thiocyanomethylthiobenzothiazol (TCMTB). We have studied separately the influence of pH, temperature and ultraviolet irradiation in aqueous...
R Perraud, M Papazian


FTIR characterization of copper ethanolamine - wood interaction
1999 - IRG/WP 99-20154
The interaction of copper ethanolamine (Cu-EA) and wood components was studied by using Fourier transform infrared spectroscopy (FTIR). In Cu-EA treated wood, significant reduction was noticed on the band attributed to carbonyl vibration from carboxylic groups at 1735±5 cm-1 and an increase in band intensity was obtained from carbonyl in carboxylate at 1595±5 cm-1. The same observation was made ...
Jun Zhang, D P Kamdem


The examination of preservative-treated radiata pine using electron spin resonance spectroscopy
1987 - IRG/WP 3423
Electron spin resonance spectroscopy (ESR) may be used to characterise the molecular environment of chemical species containing unpaired electrons. These species include free radicals, bi-radicals, point defects in solids, localised crystal imperfections and many transition metal ions. ESR has previously been applied to the examination of wood weathering or wood pyrolysis and now FRI and Massey Un...
D V Plackett, E W Ainscough, A M Brodie


Protection of cellulose string with biocide/water repellent mixtures
1997 - IRG/WP 97-30148
Protecting cellulosic materials in soil contact poses a formidable challenge. Many soil inhabiting bacteria and fungi that cannot degrade wood can nevertheless rapidly depolymerize cellulose once its protective lignin matrix is removed. As a result, cellulose based materials are rarely used in direct contact with soil. One exception, however, is the use of cellulose string to support climbing vine...
A B Chang, J J Morrell


Defining fungal decay types - A proposal for discussion
1985 - IRG/WP 1264
The present definitions of the fungal decay types, white rot, brown rot, and soft rot are somewhat vague. The definitions are based on a mixture of criteria involving morphological and chemical aspects of decay as well as taxonomic affiliation of the responsible fungal species. The present vague definitions have led some fungi being erroneously places in a wrong decay type. Furthermore, some fungi...
T Nilsson


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


The resistance of wood coated with different water-borne paints against colonisation by decay fungi
1996 - IRG/WP 96-10165
The susceptibility of wood painted with model paints of known composition to decay fungi was tested without previous weathering. Included in the study were five alkyd emulsion paints and five acrylic paints; one linseed oil paint and two solvent-borne alkyd paints. It was found that several components influenced the susceptibility of these paints. The results of the present study indicate that the...
J Bjurman


The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley


The use of X-ray diffraction for analyzing biomodification of crystalline cellulose by wood decay fungi
2007 - IRG/WP 07-10622
X-ray diffraction (XRD) is based on the creation of an interference pattern by x-rays when they encounter a regularly spaced matrix. In wood, this process has been used to determine, among other things, the average width of the cellulose microcrystals, the percent of crystalline cellulose within the wood, and can be used to examine the changes in these parameters during degradation. Enhanced under...
C Howell, A C Steenkjær Hastrup, J Jellison


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