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Treatment and recycle of CCA hazardous waste
1993 - IRG/WP 93-50007
Chromated copper arsenate containing hazardous materials is generated from the manufacture, treatment of the wood, and from the wood itself, after its life cycle. Laboratory treating of these wastes has resulted in materials suitable for recycle or disposal as non-hazardous residues. The extraction, by both acidic and ammoniacal routes, of CCA production and treating plant waste materials has been...
E A Pasek, C R McIntyre


Detecting fungal DNA in treated and non-treated wood
2007 - IRG/WP 07-10621
Isolating fungi from wood has long involved culturing on selective media followed by identification using various keys. This process can be cumbersome, costly, and, most importantly, not always capable of detecting all of the fungi present. The recent development of molecular methods for isolation and identification of fungi has created tremendous opportunities for expanding our knowledge of the...
C Freitag, M Freitag, J Morrell


Quantitative Determination of Cyproconazole in Wood treated with Tanalith CY
2007 - IRG/WP 07-20369
The HPLC method has been widely applied to quantitative determination of cyproconazole in preservative solutions. However, HPLC assay has not been successfully employed to determine cyproconazole content in treated wood. Some extractives from treated wood hinder cyproconazole determination because they have a similar retention time to that of cyproconazole on an HPLC chromatogram. In addition, ...
I Momohara, T Miyauchi, M Mori


A step towards a better understanding of fungal colonization of modified wood - QRT-PCR studies
2008 - IRG/WP 08-10653
The area of wood protection is in a period of change. New wood protection systems have been developed while their mode of action remains insufficiently understood. The development of molecular methods provides potential tools to investigate the interaction between modified wood and decay fungi. One small step to tackle some of the unsolved questions about the mode of action of modified wood is tak...
G Alfredsen, A Pilgård, A Hietala


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


Rapid Microwave-Assisted Acid Extraction of Metals from Chromated Copper Arsenate (CCA)-Treated Southern Pine Wood
2009 - IRG/WP 09-50262
The effects of acid concentration, reaction time, and temperature in a microwave reactor on recovery of CCA-treated wood were evaluated. Extraction of copper, chromium, and arsenic metals from chromated copper arsenate (CCA)-treated southern pine wood samples with three different acids (i.e., acetic acid, oxalic acid, and phosphoric acid) was investigated using in microwave reactor. Oxalic acid wa...
Bin Yu, Chung Y Hse, T F Shupe


Factors affecting sodium hypochlorite extraction of CCA preservative components from out of service treated wood for recycling
2009 - IRG/WP 09-50263
Significant amounts of chromated copper arsenate (CCA) treated wood products such as utility poles and residential constructions remain in service. There is an increasing public concern about environmental contamination from CCA treated wood when it is removed from service for reuse or recycling, placed in landfills or burned in commercial incinerators. In this paper, we investigate the effects of...
E D Gezer, P A Cooper


Detection of Wood Destroying Fungi Using DNA Microarray Technology
2010 - IRG/WP 10-20435
Wood decay fungi of the phylum Basidiomycota cause serious damage to wooden constructions and building elements. The elimination and the appraisement of fungal decay require an assured species identification. Conventional fungal diagnostics are mainly based on morphological characteristics by macro- and microscopy analysis. For some years, standardised and highly sensitive molecular methods focuss...
K Jacobs, N Rangno, W Scheiding, B Weiss, D Müller, C Hiller, W Brabetz


DNA-based tools for rapidly detecting, quantifying and monitoring ophiostomatoid fungi on beetles, in trees and wood products
2010 - IRG/WP 10-20450
Approximately half of the trees harvested for commercial purposes are lost because of native or introduced insects or insect-vectored microorganisms. Ophiostomatoid fungi, which are well adapted to dissemination by insects, include ~140 species of saprobes and pathogens. They are present worldwide, have high economical impact and many are subject to quarantine regulation. Thus, it is necessary to ...
L Khadempour, Young Woon Lim, S Massoumi Alamouti, C Breuil


Microwave-Assisted Organic Acids Extraction of Chromate Copper Arsenate (CCA)-Treated Southern Pine
2010 - IRG/WP 10-50267
The extraction effects of acid concentration, reaction time and temperature in a microwave reactor on recovery of CCA-treated wood were evaluated. Extraction of copper, chromium, and arsenic metals from chromated copper arsenate (CCA)-treated southern pine wood samples with two different organic acids (i.e., acetic acid and oxalic acid) was investigated using a microwave reactor. Oxalic acid was e...
Bin Yu, Chung Y Hse, T F Shupe


The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Investigating extraction of bifenthrin from composite wood products
2011 - IRG/WP 11-20467
Bifenthrin is added to resin prior to lay-up of plywood and laminated veneer lumber to provide termite protection. Analysis of this chemical in resins can be challenging. The potential for using ground wood in place of sawn material to recover bifenthrin was investigated using laminated veneer lumber composed of Douglas-fir veneers analyzed by three laboratories. Although the results differed amo...
J J Morrell, L Bell, J Norton, C Shaw


Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and brown rot fungi are also largely responsible for the destructive decay of wooden structures. The aim of this study was to compare two commonly used strains of Postia placenta – MAD-698-R and FPRL 280. Scots pine sapwood samples were exposed for two and eight weeks to both fungal strains. The following was inv...
N Thaler, G Alfredsen, C G Fossdal


Analyses of premature failure of utility poles
2012 - IRG/WP 12-40584
In this study a total number of 18 utility poles of Scots pine (Pinus sylvestris) impregnated with a copper-chromium containing preservative were investigated. They were part of different lower voltage transmission lines in the western part of Germany and failed before predicted minimum service life. All poles in this study were less than 15 years in use. The type of decay and fungi were evaluated...
S Bollmus, N Rangno, H Militz, A Gellerich


The effects of acetylation level on the growth of Postia placenta over 36 weeks
2012 - IRG/WP 12-40589
Genomic sequencing gives us a tool to systematically and rapidly discover novel genes, how their products function in the cell, and explore their interactions. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by different wood...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Biodegration of treated wood waste by native fungal communities of tropical soil in French Guiana
2012 - IRG/WP 12-50285
Woods have been protected with fungicides for a long time, and the effects of these fungicides on soil after being leached into the ground have turned out to be a true environmental issue. It is in this perspective that we are proposing to study fungal communities of these contaminated woods in a purpose of bioremediation. Most of precedent studies have focused on ability of some Basidiomycetes an...
A Zaremski, L Gastonguay, C Zaremski, F Chaffannel, J Beauchêne, G LeFloch


Characterization of test fields
2013 - IRG/WP 13-20508
Test field characteristics and impact of test fields on wood degradation is important when testing wood protection. The current EN 252 standard has no requirement for knowledge of decay hazards, but most commonly a test field is known as a “brown”,- “white”,- or “soft rot” field. To understand which decay hazard wood preservatives are tested against, each test field should be character...
U Råberg, N Terziev, G Daniel


Removal of nano- and micronized-copper from treated wood by chelating agents
2013 - IRG/WP 13-50294
Micronized and nano-copper (Cu)-based and arsenic and chromium-free systems have received much attention for wood protection in recent years. Because they have different fixation, and micro-distribution properties, such copper systems may be more or less subject to release using known remediation methods than soluble forms of Cu. This study evaluated Cu recovery from wood treated with micronized- ...
S N Kartal, E Terzi, B Woodward, C A Clausen, S T Lebow


Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene


Protein extraction from wood decay fungus Postia placenta
2014 - IRG/WP 14-10827
Wood decay fungi (often distinguished as white rot and brown rot) belonging to the basidiomycetes, are common inhabitants of forest litter, where they play an important role in carbon cycling. Brown rot fungi are perhaps the most important organism involved in the degradation of wood products, and of considerable economic importance. Brown rotted wood loses strength very early because of the rapid...
A Pilgård, P Arnold, K Richter


Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow


Communities of mold fungi in moisture damaged building materials
2014 - IRG/WP 14-20542
The critical conditions needed for the development of mould and decay fungi have been modelled for different building materials. However, current knowledge of indoor microbes growing on building materials relies on culture-based methods and more advanced molecular biological techniques should be employed to study the complex microbial communities in building materials. In this paper molecular biol...
E Sohlberg, H Viitanen


Practical extraction of metals from CCA-treated wood
2014 - IRG/WP 14-50305
The extraction effects of acid concentration, reaction time and temperature in a microwave reactor on recovery of chromated copper arsenate (CCA)-treated wood were evaluated. Extraction of copper, chromium, and arsenic metals from CCA-treated southern pine wood samples with three different wood particle sizes (i.e., (1) 0.5 cm3, (2) 0.5x05x1.0 cm, and (3) 0.5x0.5x1.5 cm) was investigated using a m...
Bin Yu, Chung Y Hse, T F Shupe


Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%,...
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz


Estimation of wood degradation determined by visual inspection, mechanical testing and DNA characterisation – report after 3 years of exposure
2016 - IRG/WP 16-30700
The influence of two separate treatments, thermal modification and alkaline copper quaternary (ACQ, Silvanolin©) treatment, on fungal decay, visual disfigurement, deterioration in mechanical properties and fungal community structure was assessed on Norway spruce (Picea abies) and compared with the performance of two naturally more resistant tree species native to North America: Douglas fir (Pseud...
N Thaler, T Martinović, M Bajc, D Finžgar, H Kraigher, A Sinha, J J Morrell, M Humar


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