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Wood decay fungi from New Zealand leaky buildings – PCR identification (Part 2) and aerial spore trapping
2008 - IRG/WP 08-10649
Prior to this study, it was not know which species of decay fungi caused decay in New Zealand leaky buildings. Use of molecular biology methodology, polymerase chain reaction (PCR), and subsequent DNA sequencing, as well as classical mycological techniques based on morphology, has enabled identification of decay fungi and has provided insight into their relative importance based on isolation frequ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Effect of Preservative Treatment on Fungal Colonization of Teak, Redwood, and Western Red Cedar
2009 - IRG/WP 09-20404
Fungal flora present in preservative treated samples or non-treated samples from sapwood and heartwood of teak, western red cedar, redwood, and southern yellow pine was assessed after 6 to 18 months of exposure near Hilo, Hawaii. The objectives were to compare fungal composition and diversity between treated and non-treated samples, and to examine the use of molecular techniques for assessing fung...
Y Cabrera, C Freitag, J J Morrell


Assessment of mould growth on coated wood - methods and application
2009 - IRG/WP 09-20423
Discolouring fungi reduce the service life of coated wooden claddings in façades and increase the total cost of ownership due to shorter maintenance intervals. The project “Enhanced service life on coated wooden facades” has as its main objective to develop new methods for early prediction of durability and longer aesthetic service life of coated wooden cladding related to consumer needs and ...
L Ross Gobakken, C M Whist, O Høibø, P J Hovde, E Larnøy


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


Early detection of colonisation by blue stain and mould on modified wood using PCR technique
2010 - IRG/WP 10-10730
The aim of this research was the early detection of fungal attack on the surfaces of modified and unmodified wood after natural weathering. The investigation was carried out by molecular techniques and the development and establishment of genus/species specific PCR for important moulds and sapstain fungi was part of this work. After adaption of the method, a monitoring of the succession of Ascomyc...
A Steitz, B Schmöllerl, N Pfabigan, S Bollmus, G Grüll


Postia placenta gene expression during growth in furfurylated wood
2010 - IRG/WP 10-10734
Modified wood can provide protection against a range of wood deteriorating organisms. But we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward for the mode of action against wood decaying fungi, including inhibition of action of specific enzymes, but they still need further testing. In this study gene expression of the b...
G Alfredsen, C G Fossdal


Detection of Wood Destroying Fungi Using DNA Microarray Technology
2010 - IRG/WP 10-20435
Wood decay fungi of the phylum Basidiomycota cause serious damage to wooden constructions and building elements. The elimination and the appraisement of fungal decay require an assured species identification. Conventional fungal diagnostics are mainly based on morphological characteristics by macro- and microscopy analysis. For some years, standardised and highly sensitive molecular methods focuss...
K Jacobs, N Rangno, W Scheiding, B Weiss, D Müller, C Hiller, W Brabetz


Basidiomycete colonization of Scots pine sapwood quantified by qPCR and TGA
2011 - IRG/WP 11-10750
Scots pine (Pinus sylvestris L.) is an important softwood species in Northern Europe and is frequently used as material for various wood protection systems. In Europe, EN 113 is the standard basidiomycete laboratory durability test method, using mass loss as evaluation criteria. In this paper quantitative real-time PCR (qPCR) and thermogravimetric analysis (TGA) was used to characterize coloniza...
G Alfredsen, S Bollmus, T K Bader, K Hofstetter


Quantification of four dark colored mould fungi by real time PCR
2011 - IRG/WP 11-10754
Coated wooden claddings in building facades are widely used in the Scandinavian countries, and are often preferred to other materials. Wood is facing increasing competition from other materials that are less labor intensive at the construction site and materials with less demand for maintenance thru service life, and makes further development of wooden claddings essential. Growth of discoloring mo...
E Larnøy, L Ross Gobakken, A M Hietala


Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of ...
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz


Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and brown rot fungi are also largely responsible for the destructive decay of wooden structures. The aim of this study was to compare two commonly used strains of Postia placenta – MAD-698-R and FPRL 280. Scots pine sapwood samples were exposed for two and eight weeks to both fungal strains. The following was inv...
N Thaler, G Alfredsen, C G Fossdal


Genetic diversity and morphometric study of thirteen populations of Microcerotermes diversus (Silvestri)(Isoptera: Termitidae) in southern Iran
2012 - IRG/WP 12-10782
Microcerotermes diversus (Silvestri) (Isoptera: Termitidae), an important wood pest in Iran, causes serious economic damage to cellulosic products in buildings. To investigate the genetic diversity of this pest, 13 populations from different areas of Khuzestan province as well as two samples from Khârg Island and Bandar Abbas were collected. After DNA extraction, a fragment with 486 bp length fro...
E A Firouzabadi, B Habibpour, H Galehdari, P Shishehbor


Analyses of premature failure of utility poles
2012 - IRG/WP 12-40584
In this study a total number of 18 utility poles of Scots pine (Pinus sylvestris) impregnated with a copper-chromium containing preservative were investigated. They were part of different lower voltage transmission lines in the western part of Germany and failed before predicted minimum service life. All poles in this study were less than 15 years in use. The type of decay and fungi were evaluated...
S Bollmus, N Rangno, H Militz, A Gellerich


Biodegration of treated wood waste by native fungal communities of tropical soil in French Guiana
2012 - IRG/WP 12-50285
Woods have been protected with fungicides for a long time, and the effects of these fungicides on soil after being leached into the ground have turned out to be a true environmental issue. It is in this perspective that we are proposing to study fungal communities of these contaminated woods in a purpose of bioremediation. Most of precedent studies have focused on ability of some Basidiomycetes an...
A Zaremski, L Gastonguay, C Zaremski, F Chaffannel, J Beauchêne, G LeFloch


Characterization of test fields
2013 - IRG/WP 13-20508
Test field characteristics and impact of test fields on wood degradation is important when testing wood protection. The current EN 252 standard has no requirement for knowledge of decay hazards, but most commonly a test field is known as a “brown”,- “white”,- or “soft rot” field. To understand which decay hazard wood preservatives are tested against, each test field should be character...
U Råberg, N Terziev, G Daniel


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


Postia placenta cellulase gene expression in modified wood during incipient decay
2013 - IRG/WP 13-40626
In optimization of modified wood, it is important to understand the mode of action of the wood modification and how the fungi response to it. The aim of this study was to investigate the expression of cellulases during the first two weeks of Postia placenta exposure in acetylated, DMDHEU-treated and thermally modified as well as in untreated wood. Using real-time PCR, the gene expression patterns ...
R Ringman, A Pilgård, K Richter


Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene


Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow


Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno


Estimation of wood degradation determined by visual inspection, mechanical testing and DNA characterisation – report after 3 years of exposure
2016 - IRG/WP 16-30700
The influence of two separate treatments, thermal modification and alkaline copper quaternary (ACQ, Silvanolin©) treatment, on fungal decay, visual disfigurement, deterioration in mechanical properties and fungal community structure was assessed on Norway spruce (Picea abies) and compared with the performance of two naturally more resistant tree species native to North America: Douglas fir (Pseud...
N Thaler, T Martinović, M Bajc, D Finžgar, H Kraigher, A Sinha, J J Morrell, M Humar


Genetic characterisation of the deathwatch beetle – Call for support
2017 - IRG/WP 17-10890
The deathwatch beetle (Xestobium rufovillosum) is one of the most important pests of historical wood constructions. Further analysis of the exact nature of the feeding habits regarding diet and wood characteristics might deliver answers for controlling this pest. Also there is little known about genetic variability and population structure within the species. For analysis of infested wood and also...
M Höpken, E Melcher, J Trautner


Biodiversity of wood-decaying fungi in French Guiana sequences of the small subunit (SSU) of ribosomal (r) DNA and the two primer pairs SR6/SR10R and SR7/SR1R
2019 - IRG/WP 19-10936
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests makes it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are much better known. The purposes of this work were: a) to enhance classification by integrating new data that would bring out the impo...
C Zaremski, A Ducousso-Detrez, N Amusant, A Zaremski


Identifying the fungal community on western redcedar (Thuja plicata) wood in field tests above and in ground contact exposure: preliminary results
2019 - IRG/WP 19-10939
Western redcedar (Thuja plicata) is a high value species in the Canadian forest industry due in large part to the natural durability of its heartwood. Western redcedar heartwood contains extractives that are inhibitory to the growth of many fungi responsible for decay. In order to gain insights into which extractives are important in long term durability, and which fungi are important to decay of ...
A Dale, S Kus, R Stirling


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