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An evaluation of chemical treatments for the protection of radiata pine logs from fungal degrade
1986 - IRG/WP 3377
Busan 30, Busan 1009, Mitrol PQ375. and sodium pentachlorophenoxide (NaPCP) plus have been evaluated as antisapstain treatments for the long-term protection of debarked radiata pine logs. After 3 months' winter-spring storage, treated debarked logs had less surface and internal sapstain than 'control' logs. After 6 months' storage all logs had internal sapstain ...
J A Drysdale, M E Hedley, J A Butcher


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


Wood extractives as carbon sources for staining fungi in the sapwood of lodgepole pine and trembling aspen
1995 - IRG/WP 95-10098
Wood discoloration by sapstaining fungi reduces lumber value. In Canada, the most commonly recorded sapstaining fungi belong to the genera Ophiostoma. This work evaluated the effect that Ophiostoma piceae, Ophiostoma ainoae, and Ophiostoma piliferum used the lipids present in the sapwood of lodgepole pine (Pinus contorta Dougl.) and trembling aspen (Populus tremuloides Michx.). Wood lipids, common...
Yong Gao, C Breuil


Transformation of Ophiostoma picea and Trichoderma harzianum with green fluorescent protein (GFP)
2003 - IRG/WP 03-10477
While microbial colonization of wood is presumed to be characterized by a myriad of interactions between numerous organisms, studying these processes is often difficult owing to the opaque nature of the wood and the inability to readily distinguish among the many species colonizing the material. One method for enhancing the ability to distinguish organisms is to induce specific proteins in one or ...
Ying Xiao, L M Ciuffetti, J J Morrell


Screening results of fungicides for sapstain control on Pinus radiata
1983 - IRG/WP 3236
Thirty-two compounds were tested to determine their ability to contain the growth of stain, mould and rot organisms on fresh Pinus radiata D Don. A screening technique was employed using 35 to 50 mm diameter biscuits of Pinus radiata stemwood 10 mm thick. No compound was found to be cost effective when compared against the standard treatments of NaPCP (0.5% a.i.) plus borax (1.5%) and Captafol (0....
P J Hayward, J Duff, W Rae


Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


Bacterial brown stain on sawn timber cut from water-stored logs
1992 - IRG/WP 92-1532
Brown stains which appear on the surface of sawn radiata pine cut from water-stored logs were identified as tannin-like compounds. They are derived from bacterial breakdown of flavanoid-glucosides. When timber is sawn from infected logs, the free flavanoids migrate to the wood surface as the timber dries. There, they condense and oxidise to form permanent brown discolourations. Stains occurred on ...
M E Hedley, R Meder


Laboratory evaluation of potential antisapstain treatments for Pinus radiata
1983 - IRG/WP 3237
Twelve formulations were tested in the laboratory using a 'mini' board test for effectiveness on Pinus radiata (D. Don) against sapstain, mould and decay fungi. Three formulations, sodium pentachlorophenoxide (NaPCP) plus borax, Haipen 50 WP, and Mitrol 375, are used commercially in New Zealand. Busan 1009 (0.5% product), IWD Product A (0.2% ai), IWD Product B (0.3% ai), Fennotox...
J A Drysdale


The effect of primary blue-staining fungi on the strength properties of Scots pine sapwood
2004 - IRG/WP 04-10515
The fungi action may have influence on wood properties especially Basidiomycotina fungi causing wood decay. The investigation of influence of fungi causing mainly wood discoloration and disfigurement on wood properties is still in development process. There were determined the differences between the bending strength, the compression along the grains and impact strength of control and blue stained...
A Fojutowski


Mixtures of fungicides screened for the control of sapstain on Pinus radiata
1984 - IRG/WP 3307
Fourty nine mixtures of fungicides were evaluated in a rapid laboratory screen for the control of stain, mould and decay fungi on Pinus radiata (D.Don). The most effective mixture was thiophanate methyl plus chlorothalonil closely followed by the mixtures of thiophanate methyl plus benzisothiazolone and thiophanate methyl plus dithio-bis (benzmethylamide). Benzalkonium chloride, thiram and ziram, ...
P J Hayward, W Rae, J Duff


Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Blue stain in timber in service. Results of further IRG collaborative tests to compare different artificial weathering systems
1981 - IRG/WP 2146
The previous work described in Document No IRG/WP/286 examined 3 preservatives (1% phenyl mercury succinate - PMS, 5% pentachlorophenol - PCP and 1.5% Preventol A4 - PA4) subjected to 5 different artificial weathering systems (BAM Xenotest, Cuprinol Marr, EMPA Xenotest, Gori Atlas and PRL Marr) and after natural weathering at 5 sites (BAM, Cuprinol, EMPA, Gori and PRL). Results showed good correla...
A F Bravery, D J Dickinson


Integrated protection of freshly sawn lumber using Bacillus subtilis and selected fungicide
1997 - IRG/WP 97-10235
Bioprotection against stain fungi has tremendous potential for reducing discoloration of freshly sawn wood while decreasing chemical consumption. Unfortunately, most bioprotectants appear to be unable to consistently perform under the array of conditions to which freshly sawn wood is exposed. While research is underway to understand the nature of the inconsistent performance, a more pragmatic appr...
M E Mankowski, M Anderson, J J Morrell


Effect of nutrient regimes, temperature, pH, and wood sterilization method on performance of selected bioprotectants against wood staining fungi
1992 - IRG/WP 92-1551
The effect of nutrient regimes, incubation temperature, media pH, and wood sterilization method on performance of four potential bioprotectants (Pseudomonas putida, Pseudomonas cepacia, Bacillus subtilis, and Trichoderma harzianum) against wood staining fungi were evaluated using small ponderosa pine samples over a four week period. Incubation at 32°C resulted in slight increases in the degree of...
J J Morrell, C M Secton


Färbeverfahren zur Unterscheidung von Holzzonen mit unterschiedlichem Absorptionsvermögen, insbesondere bei Fichtenholz (Picea abies Karst.). [Staining method for distinguishing zones of wood with different absorption behaviour, particularly in spruce (Picea abies Karst.)]
1980 - IRG/WP 3163
A simple regressive staining method for crosscut wood surfaces is described. Phloroglucinol-hydrochloric acid serves as stain; flowing water and a solution of sodium carbonate are used for development and fixation of a picture showing the differences of absorption. Hydrophobic zones and, contrary to this, zones with a high water content can also be shown. Comparing green spruce wood before and dur...
S Cymorek


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Artificial weathering as an aid to assessing the effectiveness of chemicals for preventing blue stain in service - a co-operative study
1984 - IRG/WP 2215
The paper reports co-operative studies carried out within Working Group II of the International Research Group on Wood Preservation to assess the effects of different artificial weathering systems on the performance of fungicides to control blue-stain in service. It has been shown that Atlas, Xenotest and Marr equipments operated according to particular standard schedules can give essentially the ...
A F Bravery, D J Dickinson


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Characteristics of the pigments produced by sap-staining fungi
1997 - IRG/WP 97-10223
The present study was conducted to characterize the pigments produced by sap-staining fungi, Aureobasidium pullulans (de Bary) Arnaud and Ceratocystis piceae (Münch) H. and P. Sydow. The pigments isolated from tested fungi were identified as melanins by spectroscopic methods (UV and infrared spectra). The UV and the infrared spectra of the fungal pigments were similar to those of the synthetic me...
M Mori, M Takahashi


New experiences with Dry Rot in Danish buildings, heat treatment and viability tests
1989 - IRG/WP 1423
An increasing number of concealed dry rot attacks, especially in older multi-storey houses under renovation, have been detected during the last few years. A necessary consequence is a demand for cheaper methods of repair, compared to the standard method used in Denmark for many years otherwise severely attacked houses will by pulled down. A heat-treatment has been developed and tested in practice....
A P Koch, C Kjerulf-Jensen, B Madsen


The susceptibility of acetylated Pinus radiata to mould and stain fungi
1992 - IRG/WP 92-1548
The aim of this investigation was to determine the effect of 5, 10, 15 and 20 percent acetylation weight gains on the susceptibility of Pinus radiata sapwood to mould and stain fungi, and to establish if an acrylic paint, an oil-based stain and a water repellant gave superior protection from fungal growth on wood treated to 20% acetylation weight gain, compared to untreated wood. One treatment set...
R N Wakeling, D V Plackett, D R Cronshaw


The influence of staining fungi on the decay resistance of wood treated with alkylammonium compounds
1984 - IRG/WP 3308
Although found to be very effective in laboratory tests, alkylammonium compounds (AAC's) have failed to perform as well in field stake tests. Examination of leachability showed that this was not the cause. The present study investigated the possibility that staining fungi, (which have been observed to rapidly infect the field stakes), degrade the AAC wood preservative. Soil-blocks were tr...
J N R Ruddick


Effect of Pseudomonas cepacia on the activity of a mixture of wood staining fungi on ponderosa pine sapwood
1995 - IRG/WP 95-10107
There are major incentives for limiting the degree of fungal discoloration which occurs during wood processing. Most lumber producers apply prophylactic fungicides which limit microbial growth, but increasing restrictions on the use of these chemicals have encouraged the search for alternative stain prevention strategies. Among the possible strategies for stain prevention is biological control. Wh...
J J Morrell, R K Velicheti


Restrictions on the use of chlorosilanes as potential wood preservatives
1985 - IRG/WP 3345
The biological protection of wood by chemical modification with different types of chlorosilanes has been investigated. The impregnation of wood has been carried out by means of liquid phase and vapor phase treatments using pure silane and silane-solvent systems. The biological efficacy against wood rotting fungi, molds and staining fungi was determined in a number of screening trials, subsequentl...
M Stevens


A study of fungal biodeterioration in unseasoned, packaged hem-fir lumber
1978 - IRG/WP 2116
This study was undertaken to determine the extent of biodeterioration that could occur within British Columbia (BC) prior to the export of packaged Canadian Lumber Standard Hem-Fir (Tsuga heterophylla, Abies amabilis and small amounts of Abies grandis). By examining the anti-stain treatments received by the lumber and identifying and characterizing the organisms responsible for the biodeterioratio...
P W Perrin, A J Cserjesi


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