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Laboratory and field trials of novel antisapstain formulations
1997 - IRG/WP 97-30146
This document covers the results of laboratory and field trials of combinations of fungicides formulated using a patented technology (PCT NZ 96/00143). A 3 week laboratory trial that uses radiata pine (Pinus radiata D. Don) branch discs as a substrate was used to determine which combinations possessed a suitable spectrum of fungitoxicity against key sapstain, mould and decay fungi. In particular f...
R N Wakeling, P N Maynard, D R Eden, C M Chittenden, B Carpenter


Characterisations of the mode of action(s) of Methylene bisthiocyanate (MBT) in Ophiostoma floccosum
2005 - IRG/WP 05-10554
Potassium (K+) leakage, oxygen consumption, glucose depletion and level of ATP formation were determined to elucidate the potential target site(s) of MBT on the sapstain fungus O. floccosum. Treatment of O. floccosum with MBT caused an efflux of intracellular K+ ions from the cells, and the rate of leakage increased with increasing MBT concentrations up to a certain value. This observation sugge...
T Singh, B Kreber, A Stewart, M Jaspers


Study of the degradation caused by micro-organisms in Pinus sp. waterlogged wood
1989 - IRG/WP 1411
So far, the different Centers are trying the restoration and the conservation of wood structures, coming from subaquatic archeological deposits, with interest from the historic - artistic point of view. The main objective of this paper has been the determination of the decay level of Pinus sp. wood coming from a roman ship (approximately 2000 years old), where we have analyzed their physical prope...
M T De Troya, M C Escorial, J Garcia, A Cabanas


Growth of sapstain fungi in scots pine and the effect of timber ageing
1998 - IRG/WP 98-10269
A trial was initiated to study the growth of known isolates of sapstain fungi in scots pine (Pinus sylvestris) which had been naturally aged or sterilised by autoclaving or irradiation. Sawlogs cut from commercial thinnings were reduced to billet size and the exposed ends were painted with a bituminous sealant to reduce moisture loss. Some billets were inoculated after autoclaving or gamma-irradia...
N J Strong, J F Webber, R A Eaton


Intraspecific variability of durability of Wapa courbaril (Eperua grandiflora) against Antrodia sp. and Coriolus versicolor: effect of radial and height position in the stem
2004 - IRG/WP 04-10531
The variation of a lesser-used species, Eperua grandiflora attacked by brown and white rot has been examined. Trees of this specie showed differences in their behaviour against the type of rot used and also showed evidence of intra and inter tree variation. The “tree” effect is very significant concerning natural resistance. In the same way, there is variation of durability observed according ...
N Amusant, J Beauchêne, M Fournier


Colonisation and detection of New Zealand sapstain fungi
2000 - IRG/WP 00-10329
Sapstain is the bluish black discolouration in logs and timber caused in New Zealand by Sphaeropsis sapinea (Diploida pinea) and more than twenty different species of the Ophiostomataceae Family. Laboratory and field trials were conducted by inoculating radiata pine to establish staining colour and extent of colonisation with various New Zealand sapstaining fungal species. Colonisation patterns, c...
J M Thwaites, R L Farrell


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Variable tolerance of Ophiostoma spp. and Diplodia pinea to commercial antisapstain products
1998 - IRG/WP 98-10266
A recent survey of the occurrence of sapstain fungi in New Zealand, conducted at The University of Waikato, provided the opportunity to examine fungicidal tolerance amongst new isolates. It also enabled a comparison of tolerance amongst new isolates with those used in routine antisapstain screening trials at Forest Research. A rapid antisapstain laboratory disc trial was used to determine the ext...
D R Eden, C M Chittenden, B Kreber, J G Van der Waals, R N Wakeling, R L Farrell, T Harrington


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete


Antisapstain field trials of NeXgen in New Zealand
1997 - IRG/WP 97-30145
This document covers the results of summer and winter log trials of NeXgen, an antisapstain formulation containing methylene bis thiocyanate plus chlorothalonil. Log billets (20 - 25cm diameter) were stripped of bark using a commercial maul debarker and antisapstain treated within 8 hours of felling by dip application. Billets were stored in a pole barn and assessed for internal sapstain and decay...
R N Wakeling, T L Woods, D R Eden, C M Chittenden, B Carpenter


Database of sapstain fungi affecting lumber, logs and trees
2004 - IRG/WP 04-10534
Sapstain fungi discolour lumber, logs and tree sapwood and are often mistaken for moulds, which cause a superficial discoloration. Stained wood has a lower market value. Further, because stained wood products can potentially carry pathogenic fungi, such products may be refused by importing countries. Addressing these issues involves developing ways for accurately identifying staining fungi, docume...
S Lee, F de Giuli Vallverdu, S Alamouti, Jae-Jin Kim, A Uzunovic, C Breuil


Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín


A study of fungal biodeterioration in unseasoned, packaged hem-fir lumber
1978 - IRG/WP 2116
This study was undertaken to determine the extent of biodeterioration that could occur within British Columbia (BC) prior to the export of packaged Canadian Lumber Standard Hem-Fir (Tsuga heterophylla, Abies amabilis and small amounts of Abies grandis). By examining the anti-stain treatments received by the lumber and identifying and characterizing the organisms responsible for the biodeterioratio...
P W Perrin, A J Cserjesi


A microscopic study on the effect of IPBC/DDAC on growth morphology of the sapstaining fungus Ophiostoma piceae
1997 - IRG/WP 97-10191
An exploratory study was performed to probe into fungal/wood/fungicide interactions using Ophiostoma piceae, a common sapstaining fungus on unseasoned radiate pine. Treated (1% solution containing IPBC (0.07% w/w) and DDAC (0.60% w/w)) and untreated radiate pine wafers were inoculated with O. piceae and examined microscopically over a ten days incubation time. The results of the study showed that ...
Ying Xiao, B Kreber


Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez


Isolation of a gene from the melanin pathway of the sapstaining fungi Ophiostoma piceae using PCR
1997 - IRG/WP 97-10219
To prevent sapstaining fungi from discoloring wood, it is necessary to determine what factors affect the biosynthesis and characteristics of the pigment(s) and to identify the genes involved in the pathway. Using inhibitors and heterologous DNA probes from Alternaria alternata, we suggest that melanin, the pigment of Ophiostoma piceae, is produced by the dihydroxynaphthalene (DHN) pathway. Recentl...
R Eagen, J Kronstad, C Breuil


Comparison of bluestain fungi growing in vitro and in vivo
1996 - IRG/WP 96-10149
Both moulds and bluestain fungi cause serious economic losses for forestry and timber processing industries and much research is aimed at finding environmentally and economically acceptable methods of control. It is especially important to study the growth of these fungi in freshly cut wood, which has been unaltered by drying or sterilisation, and which therefore resembles the substratum they woul...
A Uzunovic, J F Webber, D J Dickinson


Biological control in termite management – the potential of nematodes and fungal pathogens
2004 - IRG/WP 04-10521
A brief overview on the options for biological control of termites is presented. Many organisms have been identified as being able to kill termites, however, we do not know their real impact on field populations of termites. Most research has focused on some entomopathogenic nematodes and the fungi Beauveria bassiana and Metarhizium anisopliae. To date, only a limited number of field studies have ...
M Lenz


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Efficacies of an insecticide and a fungicide for preventing blue-stain of Japanese red pine logs
2006 - IRG/WP 06-20329
We examined blue-stain fungi on Japanese red pine at 3 forests and 2 saw mills in Iwate Prefecture, Japan. The isolated ophiostomatoid fungi were Leptographium sp and Ophiostoma sp. There were 3 routes of the blue-stain; fungal growth from the spore or hypha stuck on the cross cut surfaces, fungal spread through the inner bark by bark beetles and fungal invasion around whorled knot. The larger ext...
H Taniuchi, T Koiwa, H Masuya, S Doi


Influence of ring width and wood density on durability of oak heartwood
2008 - IRG/WP 08-10639
Oak (Quercus sp.) is considered as one of the most durable European wood species. The most important factor influencing oak durability was extractive content. In our work, we were interested in if oak-wood durability is affected by ring-width and related density. Therefore, oak heartwood specimens made of boards with different ring widths were exposed to five different fungal species Daedelea quer...
M Humar, P Oven, F Pohleven


Wood decay fungi from New Zealand leaky buildings – PCR identification (Part 2) and aerial spore trapping
2008 - IRG/WP 08-10649
Prior to this study, it was not know which species of decay fungi caused decay in New Zealand leaky buildings. Use of molecular biology methodology, polymerase chain reaction (PCR), and subsequent DNA sequencing, as well as classical mycological techniques based on morphology, has enabled identification of decay fungi and has provided insight into their relative importance based on isolation frequ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Selección de hongos reductores de extraíbles del género Ophiostoma, mediante la prueba de opacidad Tween 80
2009 - IRG/WP 08-10677
Wood extractives cause diverse problems in the industry of the wood, the pulp and the paper; among others, they produce pitch deposition during the pulp manufacturing, generating important economic losses for the sector. Recently, to mitigate these effects, it has been proposed the biological pretreatment of chips and the solid wood with colorless fungi of different species from genus Ophiostoma. ...
P Herrera, J Navarrete, C Breuil, E Werner


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