Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
E Srebotnik, K Messner
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to superficial areas. No diffusion of enzymes into the cell wall took place in white rot. Likewise, infiltration of wood. degraded by the brown rot fungus Fomitopsis pinicola, did not indicate free diffusion of the enzyme within the cell wall. This was taken as a proof of non-ezymatic cell wall degradation in brown rot.
Keywords: ELECTRON MICROSCOPY; IMMUNOGOLD LABELLING; POST EMBEDDING TECHNIQUE; DECAY; LIGNIN PEROXIDASE; WHITE ROT; PHANEROCHAETE CHRYSOSPORIUM; BROWN ROT; FOMITOPSIS PINICOLA; ENZYMES