The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum
A-C Ritschkoff, M Rättö, L Viikari
The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extract xylanase was +70°C. The enzyme stability, after 1 h incubation, decreased sharply above +60°C and pH 6.