The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to determine nano and soluble-Cu tolerance of the test fungi and Cu depletion by the fungi. A myco-remediation process of nano and soluble-Cu-treated wood by the test fungi in liquid media is also planned to determine the effect of the ions on their remediation potential. Twelve P. vitreus and nine white rot fungal strains were tested to determine optimum growth conditions in both solid malt extract agar (MEA) or potato dextrose agar (PDA) plates and liquid (N-rich and standard) media. The growth media was also regulated to different pH degrees. Radial growth of the fungi was monitored for 12 days on the media. At the end of the incubation period, the mycelial biomass and the medium pH were also determined. Considering the growth and biomass production by the fungi, MEA at 5.6 as solid medium and the standard liquid media at 5.6 and 7 were determined as most suitable artificial media. In all cases, the media at pH 9 provided unfavorable conditions for fungal growth. P.vitreus FP71186, P.vitreus ME441, and P.vitreus P151 strains were selected for further steps of the project based on their growth rate, biomass production and their capability to lower pH levels.

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