Effect of light and ventilation condition on the rate of wood decay by the brown rot basidiomycete, Tyromyces palustris
T Suzuki, M Higaki
Effect of light and the ventilation conditions of incubation jars on the wood decay by Tyromyces palustris (Berk. et Curt.) Murr. FFPRI 0507 was investigated. Under no irradiation of light, the ventilation conditions gave extensive effect on mass loss of the test pieces when the culturing was performed with culture medium designated in Japanese Industrial Standard (JIS) A 9302 (Medium A; glucose 4.0%, malt extract 1.5%, peptone 0.3%). On the other hand, no such kind of effect for ventilation conditions was recognized under light conditions, and the wood decay by the fungus accelerated by additional light irradiation of a considerably small intensity. Next, we investigated the relationship between light and the culture medium composition during the wood decay by the fungus. It was found that almost equivalent mass loss occurred after 60-90 days of cultures when the culturings were performed under light-shield conditions with medium A, the culture medium designated in Japan Wood Preserving Association (JWPA) standard No.1 (medium B; malt extract 2.0%, peptone 1.0%), and another culture medium diluted medium B by two times (medium C). Under the irradiation of light, the mass loss on the cultures in medium B and C was markedly less than that in the same media under no irradiation conditions. These results suggested that effect of light on the wood decay by Tyromyces palustris depended on the concentration of glucose in the culture medium. Further, we also investigated the activities of several extracellular and cell wall bound enzymes in wood meal medium contained medium A. From our experimental results, the activities of cellulase (b-1,4-glucan 4-glucanohydrolase, E.C. 18.104.22.168) and mannanase (b-1,4-mannan mannanohydrolase) depended on light irradiation during the wood decay and these enzyme activities may give extensive effect on the mass loss by Tyromyces palustris.