Medium temperature creosote oil (MTC) was prepared by removing light naphthalene oil and heavy anthracene oil from the coal tar by means of fractional distillation. We conducted the effectiveness test of MTC in accordance with the JWPA method for testing effectiveness of surface coatings with preservatives against decay fungi. The soil treatment test against Serpula lacrymans was also carried out with Kanuma-soil. Preservative effectiveness of MTC was sufficient for wood against Tyromyces palustris and Serpula lacrymans. The hyphal growth of Serpula lacrymans to the treated layer with MTC from the nutrient one is suppressed in the soil treatment test.

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