Molecular analysis of the basidiomycete Coniophora puteana

IRG/WP 92-1534

H E McDowell, D Button, J W Palfreyman

Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated organisms. However the profiles for Coniophora marmorata and Coniophora arida were more like that of Coniophora puteana than other organisms analysed. Analysis, by SDS-PAGE of exoproteins indicated differences between members of the Coniophora genus but, whilst there were some intra-species differences overall profiles were similar for all isolates of Coniophora puteana tested. Some cross reactivity of the Coniophora antiserum was noted, both in Western Blotting and in enzyme immunoassay and whilst the antiserum was produced against liquid culture grown organism it was able to detect Coniophora puteana when extracted from infected wood blocks. Furthermore, unlike some other basidiomycetes analysed serum components did not bind nonspecifically to Coniophora puteana.


Keywords: CELL EXTRACTS; CONIOPHORA PUTEANA; ENZYMES; FUNGI; IMMUNOASSAY; MOLECULAR ANALYSIS; PROTEINS; SDS-PAGE; WESTERN BLOTTING

Conference: 92-05-10/15 Harrogate, England, UK


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