Results of previous field studies have indicated that the spread of Trichoderma throughout the groundline regions of the interiors of creosoted poles are adversely influenced by the presence of large populations of resident mould organisms. Failure of the control fungus to develop and colonize all of the decay susceptible groundline region means that sucessful control of decay is most unlikely. This study describes a pure culture experiment to test whether precolonization of wood blocks by either Hormoconis resinae or Paecilomyces variotii effects the subsequent biological protection of these blocks against Lentinus lepideus. The results indicate that Trichoderma can protect the wood blocks from decay by the Lentinus lepideus even when the blocks are colonised by the two mould organisms prior to being treated with the Trichoderma.

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